PSMA/FOLH1 Active Protein | AMY2A active protein

Human CellExp PSMA/FOLH1, C-His Tag, human recombinant

Cat. Num. AAA849928

• Gene Names: FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• Purity: >=95% by SDS-PAGE
• Synonyms: PSMA/FOLH1; Human CellExp PSMA/FOLH1; C-His Tag; human recombinant; AMY2A; AMY2; AMY2B; PA; AMY2A active protein

• Host: HEK293 cells
• Purity/Purification: >=95% by SDS-PAGE
• Form/Format: Lyophilized powder. Lyophilized from filtered PBS (pH7.4) with Trehalose (5%)

• Gene Source: Human
• Biological Activity: Specific activity >100 U/mg protein; One unit will catalyze the hydrolysis of one micromole of 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37 degree C.
• Reconstitution Instruction: Reconstitute in PBS at >= 100 units/mL. Further dilutions should be in the lyophilization buffer (75 mM sodium chloride, 10 mM tris chloride, 1 mM calcium chloride, pH 7.2)or a similar buffer. Calcium is important for the best activity and stability.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Related Product Information for AMY2A active protein

Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human saliva, where it begins the chemical process of digestion. Foods that contain much starch but little sugar, such as rice and potato, taste slightly sweet as they are chewed because amylase turns some of their starch into sugar in the mouth. Alpha-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides such as starch and glycogen, yielding glucose and maltose. It is being used by researchers as a barometer of the body's response to physical or psychological stress. NIH study shows that Women with high levels of alpha amylase, indicating stress, are less likely to conceive.

Product Categories/Family for AMY2A active protein

Proteins; Enzymes; Proteins & Enzymes (A-Z); Native Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 548615
• NCBI GeneID: 2346
• UniProt Accession #: Q04609; O43748; Q16305; Q541A4; Q8TAY3; Q9NP15; A4UU12; A9CB79; B7Z312; B7Z343; D3DQS5; E9PDX8
• Molecular Weight: 84kDa
• NCBI Official Full Name: Glutamate carboxypeptidase 2
• NCBI Official Synonym Full Names: folate hydrolase (prostate-specific membrane antigen) 1
• NCBI Official Symbol: FOLH1
• NCBI Official Synonym Symbols: PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• NCBI Protein Information: glutamate carboxypeptidase 2; NAALADase I; glutamate carboxylase II; glutamate carboxypeptidase II; membrane glutamate carboxypeptidase; cell growth-inhibiting gene 27 protein; folylpoly-gamma-glutamate carboxypeptidase; prostate specific membrane antigen
• UniProt Protein Name: Glutamate carboxypeptidase 2
• UniProt Gene Name: FOLH1
• UniProt Synonym Gene Names: FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA
• UniProt Entry Name: FOLH1_HUMAN

NCBI Description

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

Uniprot Description

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.17.21; Protease

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: membrane; integral to plasma membrane; cytoplasm

Molecular Function: peptidase activity; dipeptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding

Biological Process: folic acid and derivative metabolic process; proteolysis