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Testing Data (Staining of mouse thymus cells with Mouse anti Mouse CD90 (Thy 1.2))

Mouse CD90 Monoclonal Antibody | anti-CD90 antibody

MOUSE ANTI MOUSE CD90

Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Applications
Flow Cytometry, Functional Assay
Synonyms
CD90; Monoclonal Antibody; MOUSE ANTI MOUSE CD90; anti-CD90 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
F7D5
Form/Format
Purified
IgM fraction - liquid
Concentration
IgM concentration 1.0 mg/ml (varies by lot)
Sequence Length
162
Applicable Applications for anti-CD90 antibody
Cytotoxic Assays (CT), Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Fusion Partners
Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
Buffer Solution
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse thymus cells with Mouse anti Mouse CD90 (Thy 1.2))

Testing Data (Staining of mouse thymus cells with Mouse anti Mouse CD90 (Thy 1.2))
Related Product Information for anti-CD90 antibody
Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB. The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake, P et al.). Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,080 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
thymus cell antigen 1, theta
NCBI Official Symbol
Thy1
NCBI Official Synonym Symbols
T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
NCBI Protein Information
thy-1 membrane glycoprotein; Thy 1.2; thy-1 antigen
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
Thy1
UniProt Synonym Gene Names
Thy-1
UniProt Entry Name
THY1_MOUSE

NCBI Description

This gene encodes a glycoprotein that is anchored to the cell surface of thymocytes, neuronal and other cells through a glycosyl-phosphatidylinositol moiety. A soluble form of the encoded protein has also been detected in serum and cerebrospinal fluid. The encoded protein undergoes further processing to generate the mature protein which mediates cell-cell interactions to trigger downstream signaling pathways. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell adhesion

Cellular Component: focal adhesion; cell surface; endoplasmic reticulum; dendrite; cytosol; lipid raft; anchored to external side of plasma membrane; growth cone; anchored to plasma membrane; membrane; apical plasma membrane; plasma membrane; myelin sheath; external side of plasma membrane

Molecular Function: integrin binding; enzyme binding; protein kinase binding

Biological Process: focal adhesion formation; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; T cell receptor signaling pathway; cell-cell adhesion; retinal cone cell development; negative regulation of protein kinase activity; negative regulation of axonogenesis; positive regulation of T cell activation; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; negative regulation of cell migration; positive regulation of GTPase activity

Research Articles on CD90

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Product Notes

The CD90 thy1 (Catalog #AAA211988) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD90 can be used in a range of immunoassay formats including, but not limited to, Cytotoxic Assays (CT), Flow cytometry (FC/FACS). Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25. Researchers should empirically determine the suitability of the CD90 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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