Rabbit anti-Human CDH24 Polyclonal Antibody | anti-CDH24 antibody

CDH24, NT (CDH24, CDH11L, Cadherin-24) (Azide free) (HRP)

Cat. Num. AAA6284153

• Gene Names: CDH24; CDH11L
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDH24; Polyclonal Antibody; NT (CDH24; CDH11L; Cadherin-24) (Azide free) (HRP); Cadherin-24; anti-CDH24 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 781

• Applicable Applications for anti-CDH24 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDH24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-89 amino acids from the N-terminal region of human CDH24.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CDH24 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CDH24 antibody detected the CDH24 protein (arrow).)

Immunohistochemistry (IHC)

(CDH24 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDH24 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CDH24 antibody

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-24 mediate strong cell-cell adhesion.

Product Categories/Family for anti-CDH24 antibody

Antibodies; Cadherin

References

Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Katafiasz, B.J., et al. J. Biol. Chem. 278(30):27513-27519(2003). Ali, J., et al. Microcirculation 4(2):267-277(1997). Hulsken, J., et al. J. Cell Biol. 127 (6 PT 2), 2061-2069 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 32880203
• NCBI GeneID: 64403
• NCBI Accession #: NP_659422.2; NP_071923.2
• NCBI GenBank Nucleotide #: NP_659422.2
• UniProt Accession #: Q86UP0
• NCBI Official Full Name: cadherin-24 isoform 2
• NCBI Official Synonym Full Names: cadherin 24
• NCBI Official Symbol: CDH24
• NCBI Official Synonym Symbols: CDH11L
• NCBI Protein Information: cadherin-24
• UniProt Protein Name: Cadherin-24
• Protein Family: Cadherin
• UniProt Gene Name: CDH24
• UniProt Synonym Gene Names: CDH11L
• UniProt Entry Name: CAD24_HUMAN

Uniprot Description

CDH24: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-24 mediate strong cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: integral to membrane; plasma membrane; intercellular junction

Molecular Function: beta-catenin binding; calcium ion binding; alpha-catenin binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; homophilic cell adhesion