SERPIND1 active protein

Human CellExp SERPIND1, human recombinant

Cat. Num. AAA844303

• Gene Names: SERPIND1; HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: SERPIND1; Human CellExp SERPIND1; human recombinant; SerpinD1; HCF2; HLS2; HC-II; SERPIND1 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 499

• Applicable Applications for SERPIND1 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.8 nM.
• Results: Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.8 nM.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for SERPIND1 active protein

Background: Serpin D1 is also known as Heparin cofactor 2 (HCF2), Protease inhibitor leuserpin-2 (HLS2), Heparin cofactor II (HC-II), which belongs to the serpin family. HCF2 is expressed predominantly in liver. Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, SerpinD1 / HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). In a glycosaminoglycan-independent manner, serpinD1 / HC-II also inhibits chymotrypsin. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

Product Categories/Family for SERPIND1 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsCytokines; Growth Factors & Horm

NCBI and Uniprot Product Information

• NCBI GI #: 73858566
• NCBI GeneID: 3053
• NCBI Accession #: NP_000176.2
• NCBI GenBank Nucleotide #: NM_000185.3
• UniProt Accession #: P05546; Q6IBZ5; B2RAI1; D3DX34
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 55.7 kDa. The predicted N-terminus is Gly 20. DTT-reduced Protein migrates as 65-70 kDa due to glycosylation.
• NCBI Official Full Name: heparin cofactor 2
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• NCBI Official Symbol: SERPIND1
• NCBI Official Synonym Symbols: HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• NCBI Protein Information: heparin cofactor 2
• UniProt Protein Name: Heparin cofactor 2
• Protein Family: Heparin cofactor
• UniProt Gene Name: SERPIND1
• UniProt Synonym Gene Names: HCF2; HC-II; HLS2
• UniProt Entry Name: HEP2_HUMAN

NCBI Description

This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015]

Uniprot Description

SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: chemotaxis; blood coagulation

Disease: Heparin Cofactor Ii Deficiency