Goat GNL3 Polyclonal Antibody | anti-GNL3 antibody

Goat anti-GNL3 (aa115-126) Antibody

Cat. Num. AAA423333

• Gene Names: GNL3; NS; E2IG3; NNP47; C77032
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GNL3; Polyclonal Antibody; Goat anti-GNL3 (aa115-126) Antibody; Goat Anti-GNL3 Antibody; guanine nucleotide binding protein-like 3 (nucleolar); C77032; E2IG3; NNP47; NS; E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide-binding protein-like 3; novel nucleolar protein 47; nucleolar GTP-binding pr; GNL3 antibody; guanine nucleotide binding protein-like 3 (nucleolar) antibody; C77032 antibody; E2IG3 antibody; NNP47 antibody; NS antibody; E2-induced gene 3 protein antibody; estradiol-induced nucleotide binding protein antibody; guanine nucleotide-binding protein-like 3 antibody; novel nucleolar protein 47 antibody; nucleolar GTP-binding protein 3 antibody; nucleostemin antibody; GNL3 (aa115-126); anti-GNL3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: TENKAKSGKQNS
• Sequence Length: 549

• Applicable Applications for anti-GNL3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 65kDa band observed in nuclear lysates of cell line K562 (calculated MW of 62. 0kDa according to NP_055181.3). Recommended concentration: 0. 03-0.1ug/ml.
• Immunogen: Peptide with sequence C-TENKAKSGKQNS, from the internal region of the protein sequence according to NP_055181.3; NP_996561.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_055181.3; NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 03ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 45593130
• NCBI GeneID: 26354
• NCBI Accession #: NP_055181.3; NP_996561.1
• NCBI GenBank Nucleotide #: NM_014366.4
• Molecular Weight: 60,540 Da
• NCBI Official Full Name: guanine nucleotide-binding protein-like 3 isoform 1
• NCBI Official Synonym Full Names: G protein nucleolar 3
• NCBI Official Symbol: GNL3
• NCBI Official Synonym Symbols: NS; E2IG3; NNP47; C77032
• NCBI Protein Information: guanine nucleotide-binding protein-like 3
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNL3
• UniProt Synonym Gene Names: E2IG3; NS; NNP47
• UniProt Entry Name: GNL3_HUMAN

NCBI Description

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

GNL3: May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. May interact with p53/TP53 via its basic domain. Increased levels in lung tissue in cancer patients. Belongs to the MMR1/HSR1 GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Nucleolus; RNA-binding; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular space; membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: cell proliferation; positive regulation of protein sumoylation; positive regulation of telomere maintenance; regulation of cell proliferation; ribosome biogenesis and assembly