PVRL1/NECTIN1 Active Protein | NECTIN1 active protein

PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120876

• Gene Names: PVRL1; ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
• Purity: >98% as determined by SDS-PAGE
• Synonyms: PVRL1/NECTIN1; PVRL1/NECTIN1 Protein; Human; Recombinant (hFc Tag); Human CD111/Nectin-1/PVRL1 Protein (Fc Tag); CD111 Protein; CLPED1 Protein; ED4 Protein; HIgR Protein; HV1S Protein; HVEC Protein; nectin-1 Protein; OFC7 Protein; PRR Protein; PRR1 Protein; PVRR Protein; PVRR1 Protein; SK-12 Protein; poliovirus receptor-related 1 (herpesvirus entry mediator C); NECTIN1 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr334

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 31
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human PVRL1 isoform 1 (NP_002846.3) extracellular domain (Met 1-Thr 334) was was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized rh PVRL3 at 1ug/ml (100uL/well) can bind recombinant human Nectin-1 with a linear range of 0.156-5ng/mL.; 2. Immobilized NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag) at 2ug/ml (100uL/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag). The EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag)is 20-120ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized rh PVRL3 at 1 ug/ml (100 ul/well) can bind recombinant human Nectin-1 with a linear range of 0.156-5 ng/ml.)

Testing Data

(Immobilized NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag) at 2 ug/ml (100 ul/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag)is 20-120 ng/mL.)

Related Product Information for NECTIN1 active protein

Background: Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

References

Yoon M, et al. (2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8.Mata M, et al. (2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80.Haarr L, et al. (2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.

NCBI and Uniprot Product Information

• NCBI GI #: 42560237
• NCBI GeneID: 5818
• NCBI Accession #: NP_002846.3
• NCBI GenBank Nucleotide #: NM_002855.4
• UniProt Accession #: Q15223; O75465; Q2M3D3; Q9HBE6; Q9HBW2
• Molecular Weight: 57,158 Da
• NCBI Official Full Name: poliovirus receptor-related protein 1 isoform 1
• NCBI Official Synonym Full Names: poliovirus receptor-related 1 (herpesvirus entry mediator C)
• NCBI Official Symbol: PVRL1
• NCBI Official Synonym Symbols: ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
• NCBI Protein Information: poliovirus receptor-related protein 1; nectin 1; poliovirus receptor-like 1; herpesvirus Ig-like receptor; herpes virus entry mediator C; ectodermal dysplasia 4 (Margarita Island type)
• UniProt Protein Name: Poliovirus receptor-related protein 1
• Protein Family: Nectin
• UniProt Gene Name: PVRL1
• UniProt Synonym Gene Names: HVEC; PRR1; Herpesvirus entry mediator C; HveC; HIgR
• UniProt Entry Name: PVRL1_HUMAN