Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF7 Cells.)
Rabbit anti-Human Protein Kinase C Iota Polyclonal Antibody | anti-PKCi antibody
Protein Kinase C Iota (PKCi) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF7 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF7 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PKCi, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PKCi, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Mcf7 cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Mcf7 cells))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCI: an AGC protein kinase of the PKC family. A calcium-independent, phospholipid-dependent atypical protein kinase C. Is not activated by phorbolesters or diaglycerol. May play role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets.
Protein type: AGC group; Cell adhesion; Cell development/differentiation; EC 2.7.11.13; Iota subfamily; Kinase, protein; Motility/polarity/chemotaxis; Oncoprotein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: apical plasma membrane; bicellular tight junction; cell leading edge; cytosol; endosome; extracellular exosome; Golgi membrane; intercellular bridge; microtubule cytoskeleton; nucleus; plasma membrane; protein complex
Molecular Function: ATP binding; metal ion binding; phospholipid binding; protein binding; protein domain specific binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity
Biological Process: actin filament organization; cell migration; cell-cell junction organization; cellular response to insulin stimulus; cytoskeleton organization; establishment of apical/basal cell polarity; establishment or maintenance of epithelial cell apical/basal polarity; eye photoreceptor cell development; Golgi vesicle budding; negative regulation of apoptosis; negative regulation of neuron apoptosis; peptidyl-serine phosphorylation; positive regulation of endothelial cell apoptotic process; positive regulation of glucose import; positive regulation of neuron projection development; positive regulation of NF-kappaB transcription factor activity; protein amino acid phosphorylation; protein targeting to membrane; secretion; vesicle-mediated transport
Research Articles on PKCi
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Product Notes
The PKCi prkci (Catalog #AAA2032849) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Protein Kinase C Iota (PKCi) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase C Iota can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PKCi prkci for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase C Iota, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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