Flow Cytometry (FC/FACS)
(Flow cytometric analysis of anti-human PVRL1 (CD111) on human erythroleukemia cell line TF-1.)
Mouse anti-Human PVRL1/NECTIN1 Monoclonal Antibody | anti-NECTIN1 antibody
Anti-PVRL1/NECTIN1 Antibody (PE), Mouse Monoclonal
Gene Names
PVRL1; ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
PVRL1/NECTIN1; Monoclonal Antibody; Anti-PVRL1/NECTIN1 Antibody (PE); Mouse Monoclonal; CD111/Nectin-1/PVRL1 Antibody (PE); Mouse MAb; poliovirus receptor-related 1 (herpesvirus entry mediator C); Anti-CD111 Antibody; Anti-CLPED1 Antibody; Anti-ED4 Antibody; Anti-HIgR Antibody; Anti-HV1S Antibody; Anti-HVEC Antibody; Anti-nectin-1 Antibody; Anti-OFC7 Antibody; Anti-PRR Antibody; Anti-PRR1 Antibody; Anti-PVRR Antibody; Anti-PVRR1 Antibody; Anti-SK-12 Antibody; anti-NECTIN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
09
Specificity
Human CD111/Nectin-1/PVRL1
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-NECTIN1 antibody
Flow Cytometry (FC/FACS/FCM)
Immunogen
Recombinant Human CD111/Nectin-1/PVRL1 protein
Conjugation
PE
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD111/Nectin-1/PVRL1 (rh CD111/Nectin-1/PVRL1) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of anti-human PVRL1 (CD111) on human erythroleukemia cell line TF-1.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of anti-human PVRL1 (CD111) on human erythroleukemia cell line TF-1.)
Related Product Information for anti-NECTIN1 antibody
Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
References
Yoon M, et al. (2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8.Mata M, et al. (2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80.Haarr L, et al. (2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,158 Da
NCBI Official Full Name
poliovirus receptor-related protein 1 isoform 1
NCBI Official Synonym Full Names
poliovirus receptor-related 1 (herpesvirus entry mediator C)
NCBI Official Symbol
PVRL1
NCBI Official Synonym Symbols
ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
NCBI Protein Information
poliovirus receptor-related protein 1; nectin 1; poliovirus receptor-like 1; herpesvirus Ig-like receptor; herpes virus entry mediator C; ectodermal dysplasia 4 (Margarita Island type)
UniProt Protein Name
Poliovirus receptor-related protein 1
Protein Family
UniProt Gene Name
PVRL1
UniProt Synonym Gene Names
HVEC; PRR1; Herpesvirus entry mediator C; HveC; HIgR
UniProt Entry Name
PVRL1_HUMAN
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Product Notes
The NECTIN1 pvrl1 (Catalog #AAA8105493) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PVRL1/NECTIN1 Antibody (PE), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PVRL1/NECTIN1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the NECTIN1 pvrl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PVRL1/NECTIN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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