NECTIN4/Nectin 4 Active Protein | NECTIN4 active protein

NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8122110

• Gene Names: PVRL4; LNIR; PRR4; EDSS1; nectin-4
• Purity: >85% as determined by SDS-PAGE
• Synonyms: NECTIN4/Nectin 4; NECTIN4/Nectin 4 Protein; Human; Recombinant (ECD; His Tag); Human NECTIN4/PVRL4 Protein (His Tag); EDSS1 Protein; LNIR Protein; nectin-4 Protein; PRR4 Protein; PVRL4 Protein; NECTIN4 active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser349

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gly 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human NECTIN4 (NP_112178.2)(Met1-Ser349) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Human NECTIN4 His at 2ug/ml (100uL/well) can bind Human PVRL1 His, biotinylated. The EC50 of Human PVRL1 His, biotinylated is 200-800ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 222136611
• NCBI GeneID: 81607
• NCBI Accession #: NP_112178.2
• NCBI GenBank Nucleotide #: NM_030916.2
• UniProt Accession #: Q96NY8; Q96K15
• Molecular Weight: 55,454 Da
• NCBI Official Full Name: poliovirus receptor-related protein 4
• NCBI Official Synonym Full Names: poliovirus receptor-related 4
• NCBI Official Symbol: PVRL4
• NCBI Official Synonym Symbols: LNIR; PRR4; EDSS1; nectin-4
• NCBI Protein Information: poliovirus receptor-related protein 4; nectin 4; Ig superfamily receptor LNIR
• UniProt Protein Name: Poliovirus receptor-related protein 4
• Protein Family: Nectin
• UniProt Gene Name: PVRL4
• UniProt Synonym Gene Names: LNIR; PRR4
• UniProt Entry Name: PVRL4_HUMAN

NCBI Description

This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]

Uniprot Description

nectin 4: Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. Defects in PVRL4 are the cause of ectodermal dysplasia- syndactyly syndrome type 1 (EDSS1). EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating. Belongs to the nectin family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: adherens junction; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell adhesion

Disease: Ectodermal Dysplasia-syndactyly Syndrome 1