Influenza A H1N1 Neuraminidase/NA Recombinant Protein | NA recombinant protein

Influenza A H1N1 (A/California/04/2009) Neuraminidase/NA (His Tag)

Cat. Num. AAA8118659

• Purity: >90 % as determined by SDS-PAGE.
• Synonyms: Influenza A H1N1 Neuraminidase/NA; Influenza A H1N1 (A/California/04/2009) Neuraminidase/NA (His Tag); NA Protein; H1N1; NA recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 480aa
• Sequence: His36-Lys469

• Species: H1N1
• Tag: C-His
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Asp
• Protein Construction: |A DNA sequence encoding the Influenza A virus (A/California/04/2009 (H1N1)) neuraminidase (ACP41107.1) (His36-Lys469) was expressed with a vasodilator-stimulated phosphoprotein tetramerization domain at the N-terminus and a polyhistidine tag at the C-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for NA recombinant protein

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza identification, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early identification of influenza viral infection. MyBioSource has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

References

Sardet C., et al., (1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.Tse C.-M., et al., (1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant Influenza A virus (A/California/04/2009 (H1N1)) NA consists of 480 amino acids and predicts a molecular mass of 53.6kDa.; As a result of glycosylation, it migrates as an approximately 58.7kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Neuraminidase