anti-Mouse PD1 Monoclonal Antibody | anti-PD1 antibody

PD1 antibody

Cat. Num. AAA833799

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Purity: PD1 antibody was purified by affinity chromatography.
• Synonyms: PD1; Monoclonal Antibody; PD1 antibody; Monoclonal PD1; Anti-PD1; PD 1; PD-1; anti-PD1 antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: J43
• Specificity: Mouse
• Purity/Purification: PD1 antibody was purified by affinity chromatography.
• Form/Format: Liquid in PBS, PH 7.2, with 0.09% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-PD1 antibody: FC, IHC, IP
• Biological Significance: The J43 monoclonal antibody reacts with mouse PD-1, a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 and PD-L2. Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
• Preparation and Storage: Store at 4°C

Flow Cytometry (FC/FACS)

(Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster IgG (biotin) and Streptavidin (PE). Total viable cells were used for analysis.)

Related Product Information for anti-PD1 antibody

Armenian Hamster monoclonal PD1 antibody

Product Categories/Family for anti-PD1 antibody

Cell Cycle & Cell Death

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1
• UniProt Entry Name: PDCD1_HUMAN

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: signal transducer activity; protein binding

Biological Process: apoptosis; multicellular organismal development; T cell costimulation; signal transduction; humoral immune response

Disease: Systemic Lupus Erythematosus