GFR Alpha-1/GFRA1 Active Protein | GFRA1 active protein

GFR Alpha-1/GFRA1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122581

• Gene Names: Gfra1; AU042498
• Purity: >98% as determined by SDS-PAGE
• Synonyms: GFR Alpha-1/GFRA1; GFR Alpha-1/GFRA1 Protein; Mouse; Recombinant (His Tag); Mouse GFRA1/GFR alpha-1 Protein (His Tag); AU042498 Protein; GDNF family receptor alpha 1; GFRA1 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser425

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRalpha1 isoform beta (AAB86600.1) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured in a cell proliferation assay using SH?SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.4-2ug/mL in the presence of 40ng/mL Recombinant Human GDNF.; 2. Measured by its ability to bind with human GDNF in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GFRA1 active protein

Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-a¢) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.

References

Jing S, et al. (1997) GFRalpha-2 and GFRalpha-3 are two new receptors for ligands of the GDNF family. J Biol Chem. 272(52): 33111-7.Jing S, et al. (1996) GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-alpha, a novel receptor for GDNF. Cell. 85(7):1113-24.Treanor JJ, et al. (1996) Characterization of a multicomponent receptor for GDNF. Nature. 382(6586): 80-3.

NCBI and Uniprot Product Information

• NCBI GI #: 2624963
• NCBI GeneID: 14585
• Molecular Weight: Molecular Mass: 46.3 kDa; Actual Protein Molecular Mass: 55-60 kDa
• NCBI Official Full Name: GDNF receptor beta
• NCBI Official Synonym Full Names: glial cell line derived neurotrophic factor family receptor alpha 1
• NCBI Official Symbol: Gfra1
• NCBI Official Synonym Symbols: AU042498
• NCBI Protein Information: GDNF family receptor alpha-1
• Protein Family: GDNF family receptor

NCBI Description

This gene encodes a transmembrane protein that functions as the receptor for glial cell line derived neurotrophic factor (GDNF). The encoded protein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-anchored cell surface coreceptor that forms a complex with the Ret tyrosine kinase in GDNF signaling pathway. Mice lacking the encoded protein exhibit deficits in the kidneys, the enteric nervous system, and spinal motor and sensory neurons similar mice deficient in GDNF or Ret. [provided by RefSeq, Jul 2016]