GFRA1 / GFR alpha-1 Active Protein | GFRA1 active protein

Recombinant Mouse GFRA1 / GFR alpha-1 Protein (His tag)

Cat. Num. AAA2547125

• Gene Names: Gfra1; AU042498
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: GFRA1 / GFR alpha-1; Recombinant Mouse GFRA1 / GFR alpha-1 Protein (His tag); AU042498; GFRA1 active protein

• Host: HEK293 Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Ser 425
• Sequence Length: 463

• Application Notes: The secreted recombinant mouse GFRalpha1consists of 412 amino acids and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Species: Mouse
• Tag: C-His
• Bioactivity: 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. ; The ED50 for this effect is typically 0.3-1.5 ug/mL in the presence of 40ng/mL Recombinant Human GDNF.; 2. Measured by its ability to bind with human GDNF in a functional ELISA.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (330ul) be added to the vial to prepare a stock solution of 0.15mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Shipping: Blue Ice; ; Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. ; Reconstituted protein solution can be stored at 4-8°C for 2-7 days. ; Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for GFRA1 active protein

Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.

NCBI and Uniprot Product Information

• NCBI GI #: 2624963
• NCBI GeneID: 14585
• Molecular Weight: Molecular Mass: 46.3 kDa; Actual Protein Molecular Mass: 55-60 kDa
• NCBI Official Full Name: GDNF receptor beta
• NCBI Official Synonym Full Names: glial cell line derived neurotrophic factor family receptor alpha 1
• NCBI Official Symbol: Gfra1
• NCBI Official Synonym Symbols: AU042498
• NCBI Protein Information: GDNF family receptor alpha-1
• UniProt Protein Name: GDNF family receptor alpha-1
• Protein Family: GDNF family receptor
• UniProt Gene Name: Gfra1
• UniProt Synonym Gene Names: Gdnfra; Trnr1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1

NCBI Description

This gene encodes a transmembrane protein that functions as the receptor for glial cell line derived neurotrophic factor (GDNF). The encoded protein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-anchored cell surface coreceptor that forms a complex with the Ret tyrosine kinase in GDNF signaling pathway. Mice lacking the encoded protein exhibit deficits in the kidneys, the enteric nervous system, and spinal motor and sensory neurons similar mice deficient in GDNF or Ret. [provided by RefSeq, Jul 2016]

Uniprot Description

GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Receptor, misc.

Chromosomal Location of Human Ortholog: 19 D2|19 54.18 cM

Cellular Component: axon; cell soma; external side of plasma membrane; extracellular space; extrinsic to membrane; receptor complex

Molecular Function: integrin binding; receptor activity

Biological Process: anatomical structure morphogenesis; cell migration; nervous system development; neurite development; neuron differentiation; transmembrane receptor protein tyrosine kinase signaling pathway