EPOR active protein

EPOR Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122482

• Purity: >95% as determined by SDS-PAGE
• Synonyms: EPOR; EPOR Protein; Mouse; Recombinant (His Tag); Mouse EPOR Protein (His Tag); erythropoietin receptor; EPOR active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro249

• Species: Mouse
• Activity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5ug/mL in the presence of 16ng/mL Recombinant mouse EPO.; 2. Measured by its binding ability in a functional ELISA.; 3. Immobilized mouse EPOR-His at 10ug/mL (100uL/well) can bind biotinylated mouse EPO-His (Cat: 51099-M08H). The EC50 of biotinylated mouse EPO-His (Cat: 51099-M08H) is 34.5-80.6ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3)(Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EPOR active protein

Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type a- single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

References

Divoky V, et al. (2002) Mouse surviving solely on human erythropoietin receptor (EpoR): model of human EpoR-linked disease. Blood 99(10): 3873-4.Carruthers SG. (2009) A truncated erythropoietin receptor EPOR-T is associated with hypertension susceptibility. Clin Pharmacol Ther. 86(2): 134-6.Baltaziak M, et al. (2009) Relationships of P53 and Bak with EPO and EPOR in human colorectal cancer. Anticancer Res. 29(10):4151-6.

NCBI and Uniprot Product Information

• NCBI GI #: 116292186
• NCBI GeneID: 13857
• NCBI Accession #: NP_034279.3
• NCBI GenBank Nucleotide #: NM_010149.3
• UniProt Accession #: P14753; Q63852
• Molecular Weight: 55,194 Da
• NCBI Official Full Name: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: Epor
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_MOUSE

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: membrane; cell; extracellular region; integral to membrane; plasma membrane; cytosol

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity; erythropoietin receptor activity

Biological Process: heart morphogenesis; elevation of cytosolic calcium ion concentration; embryonic development; viral reproduction; heart development; cardiac muscle morphogensis; ventricular cardiac muscle morphogenesis; negative regulation of neuron apoptosis; brain development; decidualization; signal transduction