EPOR recombinant protein

Recombinant Mouse EPOR Protein (His tag)

Cat. Num. AAA2547196

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: EPOR; Recombinant Mouse EPOR Protein (His tag); Epor; EPOR recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 507

• Application Notes: The recombinant mouse EPOR comprises 236 amino acids with a predicted molecular mass of 26.2 kDa. As a result of glycosylation, the apparent mplecular mass of rmEPOR is approximately 30-35 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 25
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for EPOR recombinant protein

Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type I  single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

Description: A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 116292186
• NCBI GeneID: 13857
• NCBI Accession #: NP_034279.3
• NCBI GenBank Nucleotide #: NM_010149.3
• UniProt Accession #: P14753; Q63852
• Molecular Weight: 28,798 Da
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: Epor
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: Epor
• UniProt Synonym Gene Names: EPO-R

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 9 A3|9 7.93 cM

Cellular Component: cytoplasm; external side of plasma membrane

Molecular Function: erythropoietin receptor activity; protein binding

Biological Process: brain development; cardiac muscle morphogensis; decidualization; elevation of cytosolic calcium ion concentration; embryonic development; heart development; heart morphogenesis; negative regulation of neuron apoptosis; negative regulation of nitric oxide biosynthetic process; positive regulation of cell proliferation; signal transduction; ventricular cardiac muscle morphogenesis