Rabbit anti-Human HPC2 / CBX4 Polyclonal Antibody | anti-HPC2 antibody

Rabbit Polyclonal (IgG) to Human HPC2 / CBX4

Cat. Num. AAA243346

• Gene Names: CBX4; PC2; NBP16
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified
• Synonyms: HPC2 / CBX4; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human HPC2 / CBX4; Anti-HPC2 / CBX4 Antibody IHC-plus; CBX4; Chromobox homolog 4; Chromobox protein homolog 4; E3 SUMO-protein ligase CBX4; NBP16; NS5ATP1-binding protein 16; HPC2; Polycomb 2 homolog; PC2; Pc class 2 homolog; Human HPC2; anti-HPC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Not tested with other proteins.
• Purity/Purification: Purified
• Form/Format: PBS, pH 7.4, 0.05% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 560

• Applicable Applications for anti-HPC2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (5 ug/ml), WB (1:250)
• Target Species: Human
• Immunogen Description: Synthetic peptide
• Immunogen Type: Synthetic peptide
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-HPC2 / CBX4 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

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NCBI and Uniprot Product Information

• NCBI GI #: 55770830
• NCBI GeneID: 8535
• NCBI Accession #: NP_003646.2
• NCBI GenBank Nucleotide #: NM_003655.2
• UniProt Accession #: O00257; Q6TPI8; Q96C04; B1PJR7
• Molecular Weight: 31,987 Da
• NCBI Official Full Name: E3 SUMO-protein ligase CBX4
• NCBI Official Synonym Full Names: chromobox homolog 4
• NCBI Official Symbol: CBX4
• NCBI Official Synonym Symbols: PC2; NBP16
• NCBI Protein Information: E3 SUMO-protein ligase CBX4; Pc class 2 homolog; polycomb 2 homolog; NS5ATP1-binding protein 16; chromobox protein homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila)
• UniProt Protein Name: E3 SUMO-protein ligase CBX4
• Protein Family: Histone promoter control protein
• UniProt Gene Name: CBX4
• UniProt Synonym Gene Names: Pc2; hPc2
• UniProt Entry Name: CBX4_HUMAN

Uniprot Description

CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; SUMO ligase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; nuclear speck; PcG protein complex; nucleus

Molecular Function: protein binding; enzyme binding; single-stranded RNA binding; SUMO binding; phosphoprotein binding; chromatin binding; transcription corepressor activity; methylated histone residue binding; ligase activity

Biological Process: protein sumoylation; transcription, DNA-dependent; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis