EPHA1 active protein

EphA1 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8121963

• Gene Names: EPHA1; EPH; EPHT; EPHT1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: EphA1; EphA1 Protein; Human; Recombinant (ECD; His Tag); Human EphA1/Eph Receptor A1 Protein (His Tag; ECD); EPH Protein; EPHA9 Protein; EPHT Protein; EPHT1 Protein; EPH receptor A1; EPHA1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu547

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human EPHA1 (EAL23789.1)(Met1-Glu547) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized Human EphA1 His at 2ug/ml (100uL/well) can bind Human Ephrin-A1 Fch. The EC50 of Human Ephrin-A1 Fch is 25-90ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Immobilized Human EphA1 His at 2 ug/ml (100 ul/well) can bind Human Ephrin-A1 Fch, the EC50 of Human Ephrin-A1 Fch is 25-90 ng/mL.)

Related Product Information for EPHA1 active protein

Background: EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.

Product Categories/Family for EPHA1 active protein

Actin Dynamics Signaling Pathway

References

Flanagan JG, et al. (1998) THE EPHRINS AND EPH RECEPTORS IN NEURAL DEVELOPMENT. Annual Review of Neuroscience. 21: 309-45.Wilkinson DG (2000) Eph receptors and ephrins: Regulators of guidance and assembly. International Review of Cytology. 196: 177-244.Zhou R. (1998) The Eph family receptors and ligands. Pharmacol. 77 (3): 151-81.

NCBI and Uniprot Product Information

• NCBI GI #: 51094534
• NCBI GeneID: 2041
• Molecular Weight: 51,069 Da
• NCBI Official Full Name: EphA1
• NCBI Official Synonym Full Names: EPH receptor A1
• NCBI Official Symbol: EPHA1
• NCBI Official Synonym Symbols: EPH; EPHT; EPHT1
• NCBI Protein Information: ephrin type-A receptor 1
• Protein Family: Ephrin type-A receptor

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]