B7-1 Active Protein | CD80 active protein

B7-1 Protein, Human, Recombinant (ECD, His & hFc Tag)

Cat. Num. AAA8120432

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: B7-1; B7-1 Protein; Human; Recombinant (ECD; His & hFc Tag); Human CD80/B7-1 Protein (ECD; His & Fc Tag); B7 Protein; B7.1 Protein; BB1 Protein; CD28LG Protein; CD28LG1 Protein; LAB7 Protein; CD80 molecule; CD80 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn242

• Species: Human
• Activity: Measured by its ability to bind human CD28 in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 35
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human B7-1 (NP_005182.1) precursor was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD80 active protein

Background: The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Product Categories/Family for CD80 active protein

Cancer Drug Targets

References

Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182.1
• NCBI GenBank Nucleotide #: NM_005191.3
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.