CD36 active protein

Human CellExp CD36, human recombinant

Cat. Num. AAA844903

• Gene Names: CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: CD36; Human CellExp CD36; human recombinant; SCARB3; GP3B; GP4; Platelet Glycoprotein 4; CD36 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 472

• Applicable Applications for CD36 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 200 ug/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2 mug/mL (100 muL/well) can bind rhTSP2/His with a linear range of 0.01-1 mug/mL
• Results: Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2 mug/mL (100 muL/well) can bind rhTSP2/His with a linear range of 0.01-1 mug/mL
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CD36 active protein

Background: CD36 (Cluster of Differentiation 36), also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAbeta), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 in the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macro pinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

Product Categories/Family for CD36 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and E

NCBI and Uniprot Product Information

• NCBI GI #: 48375178
• NCBI GeneID: 948
• NCBI Accession #: NP_000063.2
• NCBI GenBank Nucleotide #: NM_000072.3
• UniProt Accession #: P16671; Q13966; Q16093; Q8TCV7; Q9BPZ8; Q9BQC2; Q9BZM8; Q9BZN3; D9IX66; D9IX67; D9IX68; D9IX69
• Molecular Weight: This protein is fused with C-terminal 6×his tag at C-terminus, has a calculated MW of 47.5 kDa expressed. The predicted N-terminus is Gly30. Protein migrates as 60-90 kDa in reduced SDS-PAGE resulting from glycosylation.
• NCBI Official Full Name: platelet glycoprotein 4 isoform 1
• NCBI Official Synonym Full Names: CD36 molecule (thrombospondin receptor)
• NCBI Official Symbol: CD36
• NCBI Official Synonym Symbols: FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• NCBI Protein Information: platelet glycoprotein 4
• UniProt Protein Name: Platelet glycoprotein 4
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: CD36
• UniProt Synonym Gene Names: GP3B; GP4; FAT; GPIIIB; GPIV
• UniProt Entry Name: CD36_HUMAN

NCBI Description

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: Golgi apparatus; extracellular space; platelet alpha granule membrane; cell surface; membrane; integral to plasma membrane; plasma membrane; phagocytic vesicle; external side of plasma membrane; lipid raft

Molecular Function: low-density lipoprotein receptor activity; transforming growth factor beta binding; low-density lipoprotein binding; lipid binding; high-density lipoprotein binding

Biological Process: positive regulation of blood coagulation; positive regulation of interleukin-12 production; cGMP-mediated signaling; phagocytosis, recognition; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of transcription factor import into nucleus; low density lipoprotein mediated signaling; sequestering of lipid; antigen processing and presentation of peptide antigen via MHC class I; platelet degranulation; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; positive regulation of cell-matrix adhesion; cell adhesion; toll-like receptor 4 signaling pathway; receptor-mediated endocytosis; platelet activation; cholesterol transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of tumor necrosis factor production; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; lipoprotein transport; toll-like receptor signaling pathway; innate immune response; lipid metabolic process; blood coagulation; positive regulation of phagocytosis, engulfment; nitric oxide mediated signal transduction; plasma membrane long-chain fatty acid transport; apoptotic cell clearance

Disease: Platelet Glycoprotein Iv Deficiency; Coronary Heart Disease, Susceptibility To, 7; Malaria, Susceptibility To