CD36 / SCARB3 recombinant protein

Recombinant Rat CD36 / SCARB3 Protein (Fc tag)

Cat. Num. AAA2547386

• Gene Names: CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CD36 / SCARB3; Recombinant Rat CD36 / SCARB3 Protein (Fc tag); CD36; CD36 / SCARB3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 472

• Application Notes: The recombinant rat CD36/Fc is a disulfide-linked homodimer. The reduced monomer comprises 651 amino acids and has a predicted molecular mass of 73.3 kDa. The apparent molecular mass of the protein is approximately 108 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD36 / SCARB3 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell type s in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.

Description: A DNA sequence encoding the rat CD36 (Gly30-Lys439) was expressed with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 14250020
• NCBI GeneID: 948
• Molecular Weight: 46,090 Da
• NCBI Official Full Name: CD36 molecule (thrombospondin receptor)
• NCBI Official Synonym Full Names: CD36 molecule
• NCBI Official Symbol: CD36
• NCBI Official Synonym Symbols: FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• NCBI Protein Information: platelet glycoprotein 4
• UniProt Protein Name: Platelet glycoprotein 4
• UniProt Gene Name: CD36
• UniProt Synonym Gene Names: GP3B; GP4; FAT; GPIIIB; GPIV

NCBI Description

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: brush border membrane; cell surface; extracellular space; Golgi apparatus; integral to plasma membrane; lipid raft; membrane; phagocytic vesicle; plasma membrane; platelet alpha granule membrane

Molecular Function: lipid binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; blood coagulation; cGMP-mediated signaling; cholesterol absorption; cholesterol transport; elevation of cytosolic calcium ion concentration; interleukin-1 beta secretion; intestinal absorption; lipoprotein transport; MyD88-dependent toll-like receptor signaling pathway; nitric oxide mediated signal transduction; platelet degranulation; positive regulation of cell-matrix adhesion; receptor internalization; receptor-mediated endocytosis; response to lipid; sensory perception of taste; sequestering of lipid; triacylglycerol transport

Disease: Coronary Heart Disease, Susceptibility To, 7; Malaria, Susceptibility To; Platelet Glycoprotein Iv Deficiency