BAFF active protein

Human CellExp BAFF, Mouse Recombinant

Cat. Num. AAA849993

• Gene Names: Tnfsf13b; BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; Tnlg7a; TNFSF20; D8Ertd387e
• Purity: >=90% by SDS-PAGE
• Synonyms: BAFF; Human CellExp BAFF; Mouse Recombinant; TNFSF13B; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4; BAFF active protein

• Host: HEK293 cells
• Purity/Purification: >=90% by SDS-PAGE
• Form/Format: Lyophilized from 0.22 um filtered solution in PBS, pH5.0. Normally trehalose is added as protectant before lyophilization.

• Gene Source: Mouse
• Endotoxin: <1.0 EU per 1 ug of protein
• Biological Activity: Immobilized Mouse BAFF at 2 ug/mL (100 uL/well) can bind Mouse BAFFR, Fc Tag with a linear range of 0.4-3 ng/mL; Measured by its binding ability in a functional ELISA
• Reconstitution Instruction: Reconstitute in sterile deionized water to a concentration of 100 ug/ml.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

Testing Data

Related Product Information for BAFF active protein

B-cell activating factor (BAFF), also known as tumor necrosis factor ligand superfamily member 13B, TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS), cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

Product Categories/Family for BAFF active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins; Proteins; Enzymes; Human cell expressed recombinant proteins Cytokines; BAFFs; Cytokines & Growth Factors (A-Z); Proteins & Enzymes (A-Z); Signaling Pathways; TNF Signaling P

NCBI and Uniprot Product Information

• NCBI GI #: 1103824256
• NCBI GeneID: 24099
• NCBI Accession #: NP_001334238.1
• NCBI GenBank Nucleotide #: NM_001347309.1
• UniProt Accession #: Q9WU72; Q7TQ58
• Molecular Weight: 32,166 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13B isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: Tnfsf13b
• NCBI Official Synonym Symbols: BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; Tnlg7a; TNFSF20; D8Ertd387e
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• UniProt Gene Name: Tnfsf13b
• UniProt Synonym Gene Names: Baff

Uniprot Description

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.