BAFF active protein

Human CellExp BAFF, human recombinant

Cat. Num. AAA844540

• Gene Names: TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• Applications: SDS-Page
• Purity: >=96%
• Synonyms: BAFF; Human CellExp BAFF; human recombinant; TNFSF13B; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4; BAFF active protein

• Host: HEK 293 cells
• Purity/Purification: >=96%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM tris, 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 266

• Applicable Applications for BAFF active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug).
• Activity (Specifications/test method): Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is typically 0.3-1.8 ng/ml in the presence of goat anti mouse IgM mu chain.
• Biological Activity: Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is typically 0.3-1.8 ng/ml in the presence of goat anti mouse IgM mu chain.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for BAFF active protein

Background: B-cell activating factor (BAFF), also known as tumor necrosis factor ligand superfamily member 13B, TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS), cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

Product Categories/Family for BAFF active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; BAFFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A

NCBI and Uniprot Product Information

• NCBI GI #: 224548983
• NCBI GeneID: 10673
• NCBI Accession #: NP_001139117.1
• NCBI GenBank Nucleotide #: NM_001145645.2
• UniProt Accession #: Q9Y275; Q6FHD6; Q7Z5J2; E0ADT7
• Molecular Weight: This protein has a calculated MW of 17.2 kDa. DTT-reduced protein migrates as a 19 kDa polypeptide.
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13B isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: TNFSF13B
• NCBI Official Synonym Symbols: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• UniProt Gene Name: TNFSF13B
• UniProt Synonym Gene Names: BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
• UniProt Entry Name: TN13B_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 13q32-q34

Cellular Component: extracellular space; perinuclear region of cytoplasm; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; B cell costimulation; B cell homeostasis; immunoglobulin secretion; positive regulation of cell proliferation; T cell costimulation; positive regulation of germinal center formation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; immune response; signal transduction