ALK4/ACVR1B Recombinant Protein | ACVR1B recombinant protein

ALK4/ACVR1B Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8118641

• Gene Names: ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
• Purity: >90 % as determined by SDS-PAGE.
• Synonyms: ALK4/ACVR1B; ALK4/ACVR1B Protein; Human; Recombinant (ECD; hFc Tag); Human ALK4/ACVR1B Protein (Fc Tag); activin A receptor; type IB; ACTRIB Protein; ACVRLK4 Protein; ALK4 Protein; SKR2 Protein; ACVR1B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 341aa
• Sequence: Met1-Glu126

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Ser24
• Protein Construction: |A DNA sequence encoding the human ACVR1B (NP_004293.1) (Met1-Glu126) was expressed with the Fc region of human IgG1 at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human TDGF1-His (Cat:10908-H08H) at 10ug/mL (100uL/well) can bind ACVR1B-Fc (Cat:10583-H02H). The EC50 of human ACVR1B-Fc (Cat:10583-H02H) is 3-20ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human TDGF1-His (Cat:10908-H08H) at 10 ug/mL (100 uL/well) can bind ACVR1B-Fc (Cat:10583-H02H), the EC50 of human ACVR1B-Fc (Cat:10583-H02H) is 3-20 ng/mL.)

Related Product Information for ACVR1B recombinant protein

ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.

References

Chen Y, et al. (2005) Developmental analysis of activin-like kinase receptor-4 (ALK4) expression in Xenopus laevis. 232(2): 393-8.J. Massagu. (1998) TGF-SIGNAL TRANSDUCTION. Annual Review of Biochemistry. 67: 753-91.

NCBI and Uniprot Product Information

• NCBI GI #: 4757720
• NCBI GeneID: 91
• NCBI Accession #: NP_004293.1
• NCBI GenBank Nucleotide #: NM_004302.4
• UniProt Accession #: P36896; Q15479; Q15480; Q15481; Q15482; B7Z5L8; B7Z5W5
• Molecular Weight: 56,807 Da
• NCBI Official Full Name: activin receptor type-1B isoform a
• NCBI Official Synonym Full Names: activin A receptor, type IB
• NCBI Official Symbol: ACVR1B
• NCBI Official Synonym Symbols: ALK4; SKR2; ACTRIB; ACVRLK4
• NCBI Protein Information: activin receptor type-1B; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2
• UniProt Protein Name: Activin receptor type-1B
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR1B
• UniProt Synonym Gene Names: ACVRLK4; ALK4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_HUMAN

NCBI Description

This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

ALK4: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.11.30; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; activin receptor complex; receptor complex

Molecular Function: activin receptor activity; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; ubiquitin protein ligase binding; growth factor binding; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: development of primary female sexual characteristics; central nervous system development; in utero embryonic development; positive regulation of erythrocyte differentiation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; activin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of activin receptor signaling pathway; hair follicle development; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; G1/S transition of mitotic cell cycle