PAF-AH active protein
Human PAF-AH
Gene Names
PAFAH1B2; HEL-S-303
Reactivity
Human
Purity
> 95% by SDS-PAGE & HPLC analyses
Synonyms
PAF-AH; Human PAF-AH; PAF-acetylhydrolase; PAF 2-acylhydrolase; LDL-PLA82); 2-acetyl-1-alkylglycerophosphacholine esterase; PAF-AH active protein
Host
HEK 293 Cells
Reactivity
Human
Purity/Purification
> 95% by SDS-PAGE & HPLC analyses
Form/Format
Lyophilized
Sequence
FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGC TDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL G THWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRT LYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SW LYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNAL DL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIA LDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKE R KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSN KASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN
Sequence Length
202
Biological Activity
Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 ug/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
Related Product Information for PAF-AH active protein
Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.
Product Categories/Family for PAF-AH active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,569 Da
NCBI Official Full Name
platelet-activating factor acetylhydrolase IB subunit beta isoform b
NCBI Official Synonym Full Names
platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)
NCBI Official Symbol
PAFAH1B2
NCBI Official Synonym Symbols
HEL-S-303
NCBI Protein Information
platelet-activating factor acetylhydrolase IB subunit beta; PAFAH subunit beta; PAF-AH subunit beta; PAF-AH 30 kDa subunit; PAF-AH1b alpha 2 subunit; PAF acetylhydrolase 30 kDa subunit; epididymis secretory protein Li 303; intracellular platelet-activating factor acetylhydrolase alpha 2 subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa)
UniProt Protein Name
Platelet-activating factor acetylhydrolase IB subunit beta
UniProt Gene Name
PAFAH1B2
UniProt Synonym Gene Names
PAFAHB; PAF-AH 30 kDa subunit
UniProt Entry Name
PA1B2_HUMAN
NCBI Description
Platelet-activating factor acetylhydrolase (PAFAH) inactivates platelet-activating factor (PAF) into acetate and LYSO-PAF. This gene encodes the beta subunit of PAFAH, the other subunits are alpha and gamma. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2014]
Uniprot Description
Function: Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure: Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.
Subcellular location: Cytoplasm.
Tissue specificity: Ubiquitous. Ref.1
Sequence similarities: Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
Research Articles on PAF-AH
Similar Products
Product Notes
The PAF-AH pafah1b2 (Catalog #AAA691937) is an Active Protein produced from HEK 293 Cells and is intended for research purposes only. The product is available for immediate purchase. The Human PAF-AH reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGC TDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL G THWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRT LYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SW LYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNAL DL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIA LDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKE R KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSN KASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN. It is sometimes possible for the material contained within the vial of "PAF-AH, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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