Western Blot (WB)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Placenta Tissue.)
Rabbit anti-Human Nitric Oxide Synthase 3, Endothelial (NOS3) Polyclonal Antibody | anti-NOS3 antibody
Polyclonal Antibody to Nitric Oxide Synthase 3, Endothelial (NOS3)
MGHHHHHHSGSEF-RKMFQ ATIRSVENLQ SSKSTRATIL VRLDTGGQEG LQYQPGDHIG VCPPNRPGLV EALLSRVEDP PAPTEPVAVE QLEKGSPGGP PPGWVRDPRL PPCTLRQALT FFLDITSPPS PQLLRLLSTL AEEPREQQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Placenta Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Human Placenta Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NOS3, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NOS3, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
NCBI and Uniprot Product Information
NCBI Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Uniprot Description
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane
Molecular Function: actin monomer binding; arginine binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; protein binding; tetrahydrobiopterin binding
Biological Process: angiogenesis; arginine catabolic process; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of platelet activation; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of blood vessel diameter; positive regulation of blood vessel endothelial cell migration; positive regulation of guanylate cyclase activity; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vasodilation
Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic
Research Articles on NOS3
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Product Notes
The NOS3 nos3 (Catalog #AAA2001578) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nitric Oxide Synthase 3, Endothelial (NOS3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nitric Oxide Synthase 3, Endothelial (NOS3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-RKMFQ ATIRSVENLQ SSKSTRATIL VRLDTGGQEG LQYQPGDHIG VCPPNRPGLV EALLSRVEDP PAPTEPVAVE QLEKGSPGGP PPGWVRDPRL PPCTLRQALT FFLDITSPPS PQLLRLLSTL AEEPREQQ. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase 3, Endothelial (NOS3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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