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Heat Shock Protein 60, Rat Recombinant Protein

Heat Shock Protein 60, Recombinant, Rat (HSP60, GroEL, Chaperonin 60, cpn60)

Applications
Western Blot
Purity
Highly Purified
90% as determined by SDS-PAGE analysis.
Synonyms
Heat Shock Protein 60; Rat; Recombinant; Rat (HSP60; GroEL; Chaperonin 60; cpn60); Rat recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Highly Purified
90% as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 1mM DTT, 0.1mM PMSF.
Applicable Applications for Heat Shock Protein 60, Rat recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot analysis. If this protein is to be used for Western Blot analysis, we recommend that the material be diluted into an appropriate amount of SDS-PAGE sample buffer., On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot with an appropriate alkaline phosphatase conjugated secondary antibody for detection. This protein does not contain E. coli GroEL protein as assessed by immunoblot analysis.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at-70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for Heat Shock Protein 60, Rat recombinant protein
The rat Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65kD major antigen of mycobacteria. In eukaryotes, Hsp60 is
Product Categories/Family for Heat Shock Protein 60, Rat recombinant protein

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Product Notes

The Heat Shock Protein 60, Rat (Catalog #AAA637442) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Protein 60, Rat can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot analysis. If this protein is to be used for Western Blot analysis, we recommend that the material be diluted into an appropriate amount of SDS-PAGE sample buffer., On a 15-well minigel system, 100ng of protein per lane should be sufficient when used in a colorimetric Western Blot with an appropriate alkaline phosphatase conjugated secondary antibody for detection. This protein does not contain E. coli GroEL protein as assessed by immunoblot analysis. Researchers should empirically determine the suitability of the Heat Shock Protein 60, Rat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 60, Rat, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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