Heat Shock Protein 47 Recombinant Protein | hsp47 recombinant protein
Heat Shock Protein 47, Recombinant, Human (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antigen RA-A47, SERPINH2)
Applications
Western Blot
Purity
Highly Purified90% SDS-PAGE. Purified under denaturing conditions from the inclusion body fraction.Concentration: ~1mg/ml
Synonyms
Heat Shock Protein 47; Recombinant; Human (HSP47; Colligin; gp46; AsTP3; CBP1; CBP2; Collagen-binding protein 1; Collagen-binding protein 2 precursor; Colligin 1; Colligin 2; HSP47; arthritis-related antigen RA-A47; SERPINH2); hsp47 recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
90% SDS-PAGE. Purified under denaturing conditions from the inclusion body fraction.Concentration: ~1mg/ml
90% SDS-PAGE. Purified under denaturing conditions from the inclusion body fraction.Concentration: ~1mg/ml
Form/Format
Supplied as a liquid in 30mM Tris, pH 8.0, 100mM sodium chloride, 1mM DTT, 0.4M arginine.
Applicable Applications for hsp47 recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: We recommend that the material be diluted in an appropriate volume of an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 227: 680-685) and heated at 100 degree C for 5 min. prior to use. On a 15-well minigel system, 10ng of protein per lane should be sufficient.
Dilution: Western Blot: We recommend that the material be diluted in an appropriate volume of an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 227: 680-685) and heated at 100 degree C for 5 min. prior to use. On a 15-well minigel system, 10ng of protein per lane should be sufficient.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at-70 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for hsp47 recombinant protein
Hsp47 is a novel 47kD stress glycoprotein. The expression of Hsp47 is highly tissue as well as cell-specific and restricted to mostly phenotypically altered collagen-producing cells (1).
Product Categories/Family for hsp47 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 47
NCBI Official Symbol
hsp47
NCBI Protein Information
heat shock protein 47
Research Articles on hsp47
Similar Products
Product Notes
The hsp47 (Catalog #AAA638139) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Protein 47 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: We recommend that the material be diluted in an appropriate volume of an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 227: 680-685) and heated at 100 degree C for 5 min. prior to use. On a 15-well minigel system, 10ng of protein per lane should be sufficient. Researchers should empirically determine the suitability of the hsp47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 47, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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