Western Blot (WB)
Rabbit Hsp47 Polyclonal Antibody | anti-Hsp47 antibody
Hsp47 Antibody
Gene Names
Serpinh1; J6; Cbp1; Cbp2; gp46; Hsp47; BERF-1; Serpinh2
Reactivity
Human, Mouse, Rat, Chick
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Synonyms
Hsp47; Polyclonal Antibody; Hsp47 Antibody; SERPINH1; AsTP3; CBP1; CBP2; HSP47; PIG14; RA-A47; PPROM; colligen; colligin-2; SERPINH2; anti-Hsp47 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chick
Clonality
Polyclonal
Specificity
The antibody recognizes 47 kDa Hsp47 of human, mouse, rat, and chick origins. Mouse small intestine tissue lysate can be used as a positive control.
Form/Format
0.2 mg/ml Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Applicable Applications for anti-Hsp47 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
Recommended Dilution:
Western blotting (0.5-4 ug/ml)
Immunohistochemistry (2.5 ug/ml)
Immunoprecipitation (10-20 ug/ml)
The optimal concentrations should be determined individually.
Blocking peptide (Cat. # MBS843708) is also available.
Western blotting (0.5-4 ug/ml)
Immunohistochemistry (2.5 ug/ml)
Immunoprecipitation (10-20 ug/ml)
The optimal concentrations should be determined individually.
Blocking peptide (Cat. # MBS843708) is also available.
Immunogen
Synthetic peptide surrounding amino acid 407 of human HSP47 (ID# BV-341).
Preparation and Storage
Store at -20°C. For long term storage, aliquot and freeze at –70°C. Avoid repeated freeze/defrost cycles.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-Hsp47 antibody
Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. Significantly, HSP47 plays a vital role in folding and assembling collagen. HSP47 is also known as colligin 1, serpinh1, collagen-binding protein 1 (CBP1) and gp46. The human HSP 47 gene encodes a 47 kDa protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
serpin H1
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade H, member 1
NCBI Official Symbol
Serpinh1
NCBI Official Synonym Symbols
J6; Cbp1; Cbp2; gp46; Hsp47; BERF-1; Serpinh2
NCBI Protein Information
serpin H1
UniProt Protein Name
Serpin H1
UniProt Gene Name
Serpinh1
UniProt Synonym Gene Names
Cbp1; Hsp47; Colligin
UniProt Entry Name
SERPH_MOUSE
Uniprot Description
SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.
Cellular Component: extracellular space; endoplasmic reticulum; cytoplasm; ER-Golgi intermediate compartment
Molecular Function: serine-type endopeptidase inhibitor activity; collagen binding; protein binding; unfolded protein binding
Biological Process: collagen fibril organization; protein maturation; collagen biosynthetic process
Research Articles on Hsp47
Similar Products
Product Notes
The Hsp47 serpinh1 (Catalog #AAA840245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp47 Antibody reacts with Human, Mouse, Rat, Chick and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp47 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended Dilution: Western blotting (0.5-4 ug/ml) Immunohistochemistry (2.5 ug/ml) Immunoprecipitation (10-20 ug/ml) The optimal concentrations should be determined individually. Blocking peptide (Cat. # MBS843708) is also available. Researchers should empirically determine the suitability of the Hsp47 serpinh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp47, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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