Rabbit anti-Human HDAC8 Polyclonal Antibody | anti-HDAC8 antibody

HDAC8 (Histone Deacetylase 8, HD 8, HD8, CDA07, Histone Deacetylase-like 1, HDACL1, CDA07, RPD3)

Cat. Num. AAA648181

• Gene Names: HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• Reactivity: Human
• Applications: Immunoprecipitation
• Purity: Serum; Serum
• Synonyms: HDAC8; Polyclonal Antibody; HDAC8 (Histone Deacetylase 8; HD 8; HD8; CDA07; Histone Deacetylase-like 1; HDACL1; RPD3); Anti -HDAC8 (Histone Deacetylase 8; anti-HDAC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HDAC8.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid.
• Sequence: MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV

• Applicable Applications for anti-HDAC8 antibody: Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation.
• Immunogen: Full length human HDAC8, aa1-377 (NP_060956.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunoprecipitation (IP)

(Immunoprecipitation of HDAC8 transfected lysate using HDAC8 rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with HDAC8 mouse polyclonal antibody.)

Related Product Information for anti-HDAC8 antibody

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.

Product Categories/Family for anti-HDAC8 antibody

Antibodies; Abs to Histones

NCBI and Uniprot Product Information

• NCBI GI #: 8923769
• NCBI GeneID: 55869
• NCBI Accession #: NP_060956.1
• NCBI GenBank Nucleotide #: NM_018486.2
• UniProt Accession #: Q9BY41; Q86VC8; Q9NP76; Q9NYH4; A6ND12; A6ND61; A6NET3; A6NJR3; A8MQ62; B4DKN0; B4DV22
• Molecular Weight: 41,758 Da
• NCBI Official Full Name: histone deacetylase 8 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 8
• NCBI Official Symbol: HDAC8
• NCBI Official Synonym Symbols: HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• NCBI Protein Information: histone deacetylase 8; histone deacetylase-like 1
• UniProt Protein Name: Histone deacetylase 8
• UniProt Gene Name: HDAC8
• UniProt Synonym Gene Names: HDACL1; HD8
• UniProt Entry Name: HDAC8_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. Ref.1 Ref.2 Ref.3 Ref.12 Ref.17

Catalytic activity: Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

Cofactor: Binds 1 divalent metal cation per subunit.

Enzyme regulation: Its activity is inhibited by trichostatin A (TSA), suberoylanilide hydroxamic acid (SAHA), 3-(1-methyl-4-phenylacetyl-1H-2-pyrrolyl)-N-hydroxy-2-propenamide (APHA), 4-dimethylamino-N-(6-hydroxycarbamoyethyl)benzamide-N-hydroxy-7-(4-dimethylaminobenzoyl)aminoheptanamide (MS-344), 5-(4-methyl-benzoylamino)-biphenyl-3,4'-dicarboxylic acid 3-dimethylamide 4'-hydroxyamide (CRA-A) and butyrate. Ref.18

Subunit structure: Interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv16(p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype. The PEPB2-MYH1 fusion protein also interacts with RUNX1, a well known transcriptional regulator, suggesting that the interaction with HDAC8 may participate in the conversion of RUNX1 into a constitutive transcriptional repressor. Interacts with CBFA2T3. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin). Ref.10 Ref.11 Ref.15

Subcellular location: Nucleus. Cytoplasm. Note: Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. Ref.12 Ref.13 Ref.14

Tissue specificity: Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. Ref.2 Ref.12 Ref.13 Ref.14

Post-translational modification: Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. Ref.12 Ref.18

Involvement in disease: Cornelia de Lange syndrome 5 (CDLS5) [MIM:300882]: A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. It is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17Wilson-Turner X-linked mental retardation syndrome (WTS) [MIM:309585]: A neurologic disorder characterized by severe intellectual disability, dysmorphic facial features, hypogonadism, short stature, and truncal obesity. Affected females have a milder phenotype than affected males.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16

Sequence similarities: Belongs to the histone deacetylase family. HD type 1 subfamily.