Sheep Histone Deacetylase 3 ELISA Kit | HDAC3 elisa kit

Sheep Histone Deacetylase 3 ELISA Kit

Cat. Num. AAA745693

• Gene Names: HDAC3; HD3; RPD3; RPD3-2
• Reactivity: Sheep
• Synonyms: Histone Deacetylase 3; Sheep Histone Deacetylase 3 ELISA Kit; HDAC3 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This HDAC3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep HDAC3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDAC3 elisa kit

Principle of the assay: HDAC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HDAC3 antibody and an HDAC3-HRP conjugate. The assay sample and buffer are incubated together with HDAC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HDAC3 concentration since HDAC3 from samples and HDAC3-HRP conjugate compete for the anti-HDAC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by HDAC3 from the sample, fewer sites are left to bind HDAC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HDAC3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HDAC3 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 13128862
• NCBI GeneID: 8841
• NCBI Accession #: NP_003874.2
• NCBI GenBank Nucleotide #: NM_003883.3
• Molecular Weight: 49,111 Da
• NCBI Official Full Name: histone deacetylase 3
• NCBI Official Synonym Full Names: histone deacetylase 3
• NCBI Official Symbol: HDAC3
• NCBI Official Synonym Symbols: HD3; RPD3; RPD3-2
• NCBI Protein Information: histone deacetylase 3; SMAP45
• UniProt Protein Name: Histone deacetylase 3
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC3
• UniProt Synonym Gene Names: HD3
• UniProt Entry Name: HDAC3_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC3: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1, PRDM6 and SRY. Interacts with BTBD14B. Interacts with GLIS2. Interacts (via the DNA-binding domain) with NR2C1; the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Component of the Notch corepressor complex. Interacts with CBFA2T3 and NKAP. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with and deacetylates MAPK14. Interacts with ZMYND15. Widely expressed. Belongs to the histone deacetylase family. HD type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Nuclear receptor co-regulator; Apoptosis; Transcription, coactivator/corepressor; Deacetylase; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; spindle microtubule; histone deacetylase complex; cytoplasm; plasma membrane; nucleus

Molecular Function: cyclin binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); chromatin DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor binding; transcription corepressor activity

Biological Process: circadian rhythm; negative regulation of JNK cascade; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; regulation of mitotic cell cycle; regulation of multicellular organism growth; regulation of protein stability; spindle assembly; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of cell cycle; protein amino acid deacetylation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of TOR signaling pathway; negative regulation of apoptosis