Huntingtin Antibody | anti-HD antibody

Huntingtin Antibody

Cat. Num. AAA8509735

• Gene Names: HTT; HD; IT15; LOMARS
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Huntingtin; Huntingtin Antibody; HD protein; HDH; Huntington's disease protein; Huntington's disease protein homolog; IT15; anti-HD antibody

• Reactivity: Human, Mouse, Rat
• Specificity: Huntingtin antibody detects endogenous levels of total Huntingtin protein.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 3144

• Applicable Applications for anti-HD antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; IF: 1:100-1:500; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from Internal of human Huntingtin.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Huntingtin antibody.)

References

Macdonald M., Cell 72:971-983(1993). Matsuyama N., Genomics 69:72-85(2000). Hillier L.W., Nature 434:724-731(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 90903231
• NCBI GeneID: 3064
• NCBI Accession #: NP_002102.4
• NCBI GenBank Nucleotide #: NM_002111.8
• UniProt Accession #: P42858; Q9UQB7
• Molecular Weight: 347,603 Da
• NCBI Official Full Name: huntingtin
• NCBI Official Synonym Full Names: huntingtin
• NCBI Official Symbol: HTT
• NCBI Official Synonym Symbols: HD; IT15; LOMARS
• NCBI Protein Information: huntingtin
• UniProt Protein Name: Huntingtin
• Protein Family: Huntingtin
• UniProt Gene Name: HTT
• UniProt Synonym Gene Names: HD; IT15; HD protein

NCBI Description

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2016]

Uniprot Description

May play a role in microtubule-mediated transport or vesicle function.