SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
HCVE2 recombinant protein
HCVE2 protein (His tag)
Applications
SDS-Page
Purity
> 80% pureHCVE2 protein was purified by proprietary chromatographic techniques
Synonyms
HCVE2; HCVE2 protein (His tag); E2 protein; HCVE2 recombinant protein
Host
Viral
Purity/Purification
> 80% pure
HCVE2 protein was purified by proprietary chromatographic techniques
HCVE2 protein was purified by proprietary chromatographic techniques
Form/Format
Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
Concentration
250 ug/ml (varies by lot)
Sequence
482-671 aa: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSERPY CWHYPPRPCG IVPAKSVCGP VYCFTPSPVV VGTTDRSGAP TYSWGANDTD VFVLNNTRPP LGNWFGCTWM NSTGFTKVCG APPCVIGGVG NNTLLCPTDC FRKHPEATYS RCGSGPWITP RCMVDYPYRL WHYPCTINYT IFKVRMYVGG VEHRLEAACN WTRGERCDLE DRDRSELSPL LLSTTQ
Applicable Applications for HCVE2 recombinant protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells.
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Can be stored at 4 degree C short term. For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for HCVE2 recombinant protein
Purified recombinant HCVE2 protein (His tag)
Product Categories/Family for HCVE2 recombinant protein
Similar Products
Product Notes
The HCVE2 (Catalog #AAA5304425) is a Recombinant Protein produced from Viral and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HCVE2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HCVE2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 482-671 aa: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSERPY CWHYPPRPCG IVPAKSVCGP VYCFTPSPVV VGTTDRSGAP TYSWGANDTD VFVLNNTR PP LGNWFGCTWM NSTGFTKVCG APPCVIGGVG NNTLLCPTDC FRKHPEATYS RCGSGPWITP RCMVDYPYRL WHYPCTINYT IFKVRMYV GG VEHRLEAACN WTRGERCDLE DRDRSELSPL LLSTTQ. It is sometimes possible for the material contained within the vial of "HCVE2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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