DC-SIGNR/CD299 recombinant protein

Recombinant human DC-SIGNR/CD299 protein

Cat. Num. AAA201311

• Gene Names: CLEC4M; CD299; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: DC-SIGNR/CD299; Recombinant human DC-SIGNR/CD299 protein; CD209L; DC-SIGN2; DCSIGNR; HP10347; L-SIGN; LSIGN; C-type lectin domain family 4 member M; CLEC4M; C-type lectin domain family 4 member M isoform 1; CD209L1; CD299; DC-SIGNR; CD209 antigen-like protein; DC-SIGN-related protein; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; Liver/lymph node-specific ICAM-3-grabbing non-integrin; DC-SIGNR/CD299 recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 72-399aa
• Sequence: VSKVPSS LSQEQSEQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTRLKAA VGELPEKSKL QEIYQELTRL KAAVGELPEK SKLQEIYQEL TELKAAVGEL PEKSKLQEIY QELTQLKAAV GELPDQSKQQ QIYQELTDLK TAFERLCRHC PKDWTFFQGN CYFMSNSQRN WHDSVTACQE VRAQLVVIKT AEEQNFLQLQ TSRSNRFSWM GLSDLNQEGT WQWVDGSPLS PSFQRYWNSG EPNNSGNEDC AEFSGSGWND NRCDVDNYWI CKKPAACFRD E

• Applicable Applications for DC-SIGNR/CD299 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for DC-SIGNR/CD299 recombinant protein

CD299, as known as DC-SIGNR and CLEC4M, is a type II integral membrane protein that is 77% amino acid identical to CD209/DC-SIGN, an HIV gp120-binding protein. This protein is probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. It may mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. CD299 is a receptor for ICAM3, binding to mannose-like carbohydrates. It also recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including tuberculosis mycobacteria, and viruses including Ebola, hepatitis C, HIV-1, influenza A, West Nile virus and the SARS-CoV acute respiratory syndrome coronavirus. Recombinant human CD299, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for DC-SIGNR/CD299 recombinant protein

Immunology; Proteins

References

Gijzen K., et al, (2008) Exp Hematol. 36:860-870. Johnson TR, et al, (2012) J Virol. 86:1339-1347. Xia HB, et al, (2020) Oncol Lett. 19:69-76.

NCBI and Uniprot Product Information

• NCBI GI #: 42542390
• NCBI GeneID: 10332
• NCBI Accession #: NP_055072
• NCBI GenBank Nucleotide #: NM_014257
• UniProt Accession #: Q9H2X3
• Molecular Weight: 44kDa
• NCBI Official Full Name: C-type lectin domain family 4 member M isoform 1
• NCBI Official Synonym Full Names: C-type lectin domain family 4 member M
• NCBI Official Symbol: CLEC4M
• NCBI Official Synonym Symbols: CD299; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR
• NCBI Protein Information: C-type lectin domain family 4 member M
• UniProt Protein Name: C-type lectin domain family 4 member M
• UniProt Gene Name: CLEC4M
• UniProt Synonym Gene Names: CD209L; CD209L1; CD299; DC-SIGNR; DC-SIGN2; L-SIGN
• UniProt Entry Name: CLC4M_HUMAN

NCBI Description

This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.[provided by RefSeq, Feb 2009]

Uniprot Description

CLEC4M: Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: integral to plasma membrane; membrane

Molecular Function: calcium-dependent protein binding; viral receptor activity; virion binding

Biological Process: cell-cell recognition; regulation of blood coagulation; regulation of gene expression; virion attachment to host cell surface receptor; virus-host interaction