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Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 ELISA Kit | HCN3 elisa kit

Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit

Reactivity
Human
Synonyms
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3; Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit; HCN3 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human HCN3. No significant cross-reactivity or interference between Human HCN3 and analogues was observed.
Sequence Length
774
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HCN3 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HCN3 in samples. An antibody specific for HCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,032 Da
NCBI Official Full Name
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
NCBI Official Synonym Full Names
hyperpolarization activated cyclic nucleotide gated potassium channel 3
NCBI Official Symbol
HCN3
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
UniProt Gene Name
HCN3
UniProt Synonym Gene Names
KIAA1535

NCBI Description

This gene encodes a multi-pass membrane protein that functions as a voltage gated cation channel. The encoded protein is a member of a family of closely related cyclic adenosine monophosphate-binding channel proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

HCN3: Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Belongs to the potassium channel HCN family.

Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: axon; cell soma; cone cell pedicle; dendrite; integral component of plasma membrane; plasma membrane

Molecular Function: cAMP binding; protein binding; voltage-gated potassium channel activity; voltage-gated sodium channel activity

Biological Process: cellular response to cAMP; potassium ion transmembrane transport; regulation of ion transmembrane transport; regulation of membrane potential

Research Articles on HCN3

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Product Notes

The Human HCN3 hcn3 (Catalog #AAA282244) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282244 ELISA Kit recognizes Human HCN3. It is sometimes possible for the material contained within the vial of "Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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