Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) Standard Curve.)
Rat Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 (HCN3) ELISA Kit | HCN3 elisa kit
Rat Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 (HCN3) ELISA Kit
Reactivity
Rat
Synonyms
Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 (HCN3); Rat Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 (HCN3) ELISA Kit; HCN3; KIAA1535; MGC131493; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3; HCN3 elisa kit
Reactivity
Rat
Specificity
Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HCN3. No significant cross-reactivity or interference between Rat HCN3 and analogues was observed.
Sequence Length
780
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) Standard Curve.)
Related Product Information for HCN3 elisa kit
Description: This Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit employs a two-site sandwich ELISA to quantitate HCN3 in samples. An antibody specific for HCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HCN3 conducted both potassium and sodium ions with a 3:1 preference for potassium ions. HCN3 bound cAMP, but unlike other HCNs, its activity was not modulated by intracellular cAMP.The cDNA contains a repetitive element in its 3-prime proximal region, and the deduced protein contains 703 amino acids. RT-PCR ELISA detected high HCN3 expression in adult liver and kidney and fetal liver and brain, intermediate expression in adult whole brain, lung, pancreas, spleen, testis, ovary, and all specific brain regions examined, and little to no expression in heart and skeletal muscle.Expression was high in fetal brain and adult cerebellum and intermediate in nucleus accumbens, thalamus, and pituitary gland, with little to no expression in other brain regions examined.
Background: HCN3 conducted both potassium and sodium ions with a 3:1 preference for potassium ions. HCN3 bound cAMP, but unlike other HCNs, its activity was not modulated by intracellular cAMP.The cDNA contains a repetitive element in its 3-prime proximal region, and the deduced protein contains 703 amino acids. RT-PCR ELISA detected high HCN3 expression in adult liver and kidney and fetal liver and brain, intermediate expression in adult whole brain, lung, pancreas, spleen, testis, ovary, and all specific brain regions examined, and little to no expression in heart and skeletal muscle.Expression was high in fetal brain and adult cerebellum and intermediate in nucleus accumbens, thalamus, and pituitary gland, with little to no expression in other brain regions examined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
NCBI Official Synonym Full Names
hyperpolarization-activated cyclic nucleotide-gated potassium channel 3
NCBI Official Symbol
Hcn3
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
UniProt Gene Name
Hcn3
UniProt Entry Name
HCN3_RAT
NCBI Description
may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006]
Research Articles on HCN3
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Product Notes
The Rat HCN3 hcn3 (Catalog #AAA9712575) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712575 ELISA Kit recognizes Rat HCN3. It is sometimes possible for the material contained within the vial of "Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 (HCN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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