Typical Testing Data/Standard Curve (for reference only)
Rat hemoglobin, alpha 1 ELISA Kit | HBA1 elisa kit
Rat Hemoglobin, Hb ELISA Kit
Reactivity
Rat
Synonyms
hemoglobin; alpha 1; Rat Hemoglobin; Hb ELISA Kit; Rat Hemoglobin (Hb) ELISA Kit; CD31; MGC126895; MGC126897; alpha 1 globin; alpha one globin; alpha-1 globin; alpha-1-globin; hemoglobin alpha 1 globin chain; hemoglobin alpha-1 chain; HBA1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat Hb. No significant cross-reactivity or interference between rat Hb and analogues was observed.
Sequence Length
142
Samples
Serum, plasma, Lysate for RBC
Assay Type
Competitive
Detection Range
12.5 ng/ml-800 ng/ml.
Sensitivity
The minimum detectable dose of rat Hb is typically less than 6.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat Hb concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HBA1 elisa kit
Principle of the assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for Hb has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated Hb. A competitive inhibition reaction is launched between Hb (Standards or samples) and biotin-conjugated Hb with the pre-coated Hb antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of Hb bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,329 Da
NCBI Official Full Name
hemoglobin, alpha 2
NCBI Official Synonym Full Names
hemoglobin, alpha 2
NCBI Official Symbol
Hba2
NCBI Protein Information
hemoglobin, alpha 2; 2-alpha globin; 2-alpha-1 globin; hemoglobin alpha 2 chain
UniProt Protein Name
Hemoglobin subunit alpha-1/2
Protein Family
UniProt Gene Name
Hba1
UniProt Synonym Gene Names
Hba-a1
UniProt Entry Name
HBA_RAT
Uniprot Description
Function: Involved in oxygen transport from the lung to the various peripheral tissues.
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Product Notes
The Rat HBA1 hba1 (Catalog #AAA700430) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700430 ELISA Kit recognizes Rat HBA1. It is sometimes possible for the material contained within the vial of "hemoglobin, alpha 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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