Fish Complement 3, C3 ELISA Kit | C3 elisa kit

Fish Complement 3, C3 ELISA Kit

Cat. Num. AAA705123

• Reactivity: Fish
• Synonyms: Complement 3; C3; Fish Complement 3; C3 ELISA Kit; C3 elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish C3. No significant cross-reactivity or interference between fish C3 and analogues was observed.

• Samples: Serum, plasma
• Assay Type: Competitive
• Detection Range: 12.5 ?g/ml -500 ?g/ml.
• Sensitivity: The minimum detectable dose of fish C3 is typically less than 12.5 ?g/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish C3 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C3 elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for C3 and Horseradish Peroxidase (HRP) conjugated C3. The competitive inhibition reaction is launched between with HRP labeled C3 and unlabeled C3 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of C3 in the sample. The color development is stopped and the intensity of the color is measured.