TIM-3 / HAVCR2 recombinant protein

Recombinant Human TIM-3 / HAVCR2 Protein (His tag)

Cat. Num. AAA2546289

• Gene Names: HAVCR2; TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: TIM-3 / HAVCR2; Recombinant Human TIM-3 / HAVCR2 Protein (His tag); HAVCR2; FLJ14428; KIM-3; TIM3; TIMD3; Tim-3; TIM-3 / HAVCR2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 301

• Application Notes: The recombinant human TIMD3 consists of 190 amino acids after removal of the signal peptide and has a predicted molecular mass of 21.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTIMD is approximately 40-45 kDa due to glycosylation.
• Predicted N Terminal: Ser 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TIM-3 / HAVCR2 recombinant protein

Background: Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both mice and human. Recently, identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In tumor rejection model, soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.

Description: A DNA sequence encoding the human TIMD3 (NP_116171.3) extracellular domain (Met 1-Arg 200) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 49574534
• NCBI GeneID: 84868
• NCBI Accession #: NP_116171.3
• NCBI GenBank Nucleotide #: NM_032782.4
• UniProt Accession #: Q8TDQ0; Q8WW60; Q96K94; B2RAY2
• Molecular Weight: 16,149 Da
• NCBI Official Full Name: hepatitis A virus cellular receptor 2
• NCBI Official Synonym Full Names: hepatitis A virus cellular receptor 2
• NCBI Official Symbol: HAVCR2
• NCBI Official Synonym Symbols: TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
• NCBI Protein Information: hepatitis A virus cellular receptor 2
• UniProt Protein Name: Hepatitis A virus cellular receptor 2
• UniProt Gene Name: HAVCR2
• UniProt Synonym Gene Names: TIM3; TIMD3; HAVcr-2; TIMD-3; TIM-3

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]

Uniprot Description

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: cell surface

Molecular Function: protein binding

Biological Process: inhibition of NF-kappaB transcription factor; maternal process involved in pregnancy; natural killer cell tolerance induction; negative regulation of interferon-alpha production; negative regulation of interferon-gamma production; negative regulation of interleukin-2 production; negative regulation of interleukin-3 production; negative regulation of myeloid dendritic cell activation; negative regulation of tumor necrosis factor production; positive regulation of interleukin-4 production

Disease: Rheumatoid Arthritis