HA Recombinant Protein

HA (Y98F) (A/Brisbane/02/2018) (H1N1)

Cat. Num. AAA434269

• Applications: Western Blot, ELISA
• Purity: >95% pure by 12% SDS PAGE gel
• Synonyms: HA; HA (Y98F) (A/Brisbane/02/2018) (H1N1); HA recombinant protein

• Host: 293 Cell Culture
• Purity/Purification: >95% pure by 12% SDS PAGE gel
• Concentration: 1 ug/ul in PBS (varies by lot)
• Sequence: EQKLISEEDLDTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLGGVAPLHLGKCNIAGWILGNPECESLSTARSWSYIVETSNSDNGTCYPGDFINFEELREQLSSVSSFERFEIFKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLNQTYINDKGKEVLVLWGIHHPPTTADQQSLYQNADAYVFVGTSRYSKKFKPEIATRPKVRDREGRMNYYWTLVEPGDKITFEATNLVVPRYAFTMERNAGSGIIISDTPVHDCNTTCQTAEGAINTSLPFQNVHPVTIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRNQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREKIDVKLESTRIYQIHHHHHH

• Applicable Applications for HA recombinant protein: Western Blot standard, Antibody ELISA, Antigen
• Viral Protein: Recombinant hemagglutinin (aa 18-530)(A/Brisbane/02/2018/H1N1)-pdm09-like produced in human 293 cells is a glycosylated polypeptide chain with amino acid change of Y98F, and having a C-terminal 6x his tag and an N-terminal c-Myc tag (GISAID accession#: EPI1312566)
• Endotoxin Level: <0.01 EU per 1 ug of the protein by LAL test
• Preparation and Storage: Store at -20 degree C; Stable for 6-months from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE: purified HA (Y98F)(aa 18-530)(A/Brisbane/02/2018/H1N1) protein)

Related Product Information for HA recombinant protein

Viral protein purified from 293 cell culture
Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. HA is an antigenic glycoprotein, like all other hemagglutinins, it causes red blood cells to agglutinate. HA is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.

HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA1 globule.