Human NFKB Activating Protein (NKAP) ELISA Kit | NKAP elisa kit

Human NFKB Activating Protein (NKAP) ELISA Kit

Cat. Num. AAA2025602

• Reactivity: Human
• Synonyms: NFKB Activating Protein (NKAP); Human NFKB Activating Protein (NKAP) ELISA Kit; NF Kappa B Activating Protein; NKAP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NFKB Activating Protein (NKAP).; No significant cross-reactivity or interference between NFKB Activating Protein (NKAP) and analogues was observed.
• Sequence Length: 415

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NFKB Activating Protein (NKAP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NFKB Activating Protein (NKAP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NKAP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB Activating Protein (NKAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFKB Activating Protein (NKAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB Activating Protein (NKAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NFKB Activating Protein (NKAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NKAP elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 15929864
• NCBI GeneID: 79576
• Molecular Weight: 47,138 Da
• NCBI Official Full Name: NFKB activating protein
• NCBI Official Synonym Full Names: NFKB activating protein
• NCBI Official Symbol: NKAP
• NCBI Protein Information: NF-kappa-B-activating protein
• UniProt Protein Name: NF-kappa-B-activating protein
• Protein Family: NF-kappa-B-activating protein
• UniProt Gene Name: NKAP
• UniProt Entry Name: NKAP_HUMAN

NCBI Description

This gene encodes a protein that is involved in the activation of the ubiquitous transcription factor NF-kappaB. This protein is associated with the the histone deacetylase HDAC3 and with the Notch corepressor complex, and it thereby acts as a transcriptional repressor of Notch target genes. It is also required for alphabeta T cell development. A related pseudogene has been identified on chromosome X, while a related and intronless retrocopy, which has an intact CDS and may be functional, is located on chromosome 6. [provided by RefSeq, May 2010]

Uniprot Description

NKAP: Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter. Belongs to the UPF0396 family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; chromatin DNA binding; chromatin binding

Biological Process: Notch signaling pathway; transcription, DNA-dependent; granulocyte differentiation; stem cell maintenance; T cell differentiation in the thymus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of alpha-beta T cell differentiation