Human Granzyme A ELISA Kit | GZMA elisa kit

Human Granzyme A ELISA Kit

Cat. Num. AAA761714

• Gene Names: GZMA; HFSP; CTLA3
• Reactivity: Human
• Synonyms: Granzyme A; Human Granzyme A ELISA Kit; GZMA (Granzyme A)/CTL Tryptase/CTLA3/Fragmentin-1/HF/HFSP/Cytotoxic T-lymphocyte proteinase 1/Granzyme-1/H factor/Hanukkah factor; GZMA elisa kit

• Reactivity: Human
• Sequence Length: 23

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GZMA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GZMA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GZMA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GZMA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GZMA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4096788
• NCBI GeneID: 3001
• Molecular Weight: 27,109 Da
• NCBI Official Full Name: granzyme A, partial
• NCBI Official Synonym Full Names: granzyme A
• NCBI Official Symbol: GZMA
• NCBI Official Synonym Symbols: HFSP; CTLA3
• NCBI Protein Information: granzyme A
• UniProt Protein Name: Granzyme A
• Protein Family: Granzyme
• UniProt Gene Name: GZMA
• UniProt Synonym Gene Names: CTLA3; HFSP; H factor; HF
• UniProt Entry Name: GRAA_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Apoptosis; EC 3.4.21.78; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: extracellular region; immunological synapse; nucleus

Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: apoptosis; cytolysis; immune response; negative regulation of DNA binding; negative regulation of endodeoxyribonuclease activity; negative regulation of oxidoreductase activity; positive regulation of apoptosis; proteolysis involved in cellular protein catabolic process