Human Granzyme A ELISA Kit | GZMA elisa kit

Human Granzyme A ELISA Kit

Cat. Num. AAA8248358

• Gene Names: GZMA; HFSP; CTLA3
• Reactivity: Human
• Synonyms: Granzyme A; Human Granzyme A ELISA Kit; CTLA3; HFSP; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF; GZMA elisa kit

• Reactivity: Human
• Specificity: The Human Granzyme A ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Granzyme A proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 262

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GZMA elisa kit

Principle of the Assay: The Human Granzyme A ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Granzyme A in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Granzyme A coated on a 96-well plate. Standards and samples are pipetted into the wells and Granzyme A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Granzyme A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Granzyme A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A.

NCBI and Uniprot Product Information

• NCBI GI #: 5453676
• NCBI GeneID: 3001
• NCBI Accession #: NP_006135.1
• NCBI GenBank Nucleotide #: NM_006144.3
• UniProt Accession #: P12544; Q6IB36; A4PHN1
• Molecular Weight: 27,109 Da
• NCBI Official Full Name: granzyme A
• NCBI Official Synonym Full Names: granzyme A
• NCBI Official Symbol: GZMA
• NCBI Official Synonym Symbols: HFSP; CTLA3
• NCBI Protein Information: granzyme A
• UniProt Protein Name: Granzyme A
• Protein Family: Granzyme
• UniProt Gene Name: GZMA
• UniProt Synonym Gene Names: CTLA3; HFSP; H factor; HF
• UniProt Entry Name: GRAA_HUMAN

NCBI Description

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Apoptosis; EC 3.4.21.78; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: immunological synapse; nucleus

Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: apoptosis; immune response; negative regulation of DNA binding; negative regulation of endodeoxyribonuclease activity; negative regulation of oxidoreductase activity; positive regulation of apoptosis; proteolysis involved in cellular protein catabolic process