Typical Testing Data/Standard Curve (for reference only)
Guinea Pig MYH1 ELISA Kit | MYH1 elisa kit
Guinea pig MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult) ELISA Kit
Gene Names
MYH1; MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
Reactivity
Guinea Pig
Synonyms
MYH1; Guinea pig MYH1 (Myosin Heavy Chain 1; Skeletal Muscle; Adult) ELISA Kit; MYH1 elisa kit
Reactivity
Guinea Pig
Specificity
This kit recognizes natural and recombinant Guinea pig MYH1. No significant cross-reactivity or interference between Guinea pig MYH1 and analogues was observed.
Sequence Length
1939
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MYH1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig MYH1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MYH1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MYH1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MYH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MYH1. You can calculate the concentration of MYH1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MYH1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MYH1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MYH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MYH1. You can calculate the concentration of MYH1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
223,145 Da
NCBI Official Full Name
myosin-1
NCBI Official Synonym Full Names
myosin, heavy chain 1, skeletal muscle, adult
NCBI Official Symbol
MYH1
NCBI Official Synonym Symbols
MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
NCBI Protein Information
myosin-1; epididymis luminal protein 71; myHC-IIx/d; myosin heavy chain 1; myosin heavy chain 2x; myosin heavy chain IIx/d; myosin heavy chain, skeletal muscle, adult 1; myosin, heavy polypeptide 1, skeletal muscle, adult
UniProt Protein Name
Myosin-1
UniProt Gene Name
MYH1
UniProt Synonym Gene Names
MyHC-2x; MyHC-IIx/d
UniProt Entry Name
MYH1_HUMAN
NCBI Description
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
Uniprot Description
MYH1: Muscle contraction.
Protein type: Motor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: muscle myosin complex; A band
Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding
Biological Process: muscle contraction; metabolic process
Research Articles on MYH1
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Product Notes
The Guinea Pig MYH1 myh1 (Catalog #AAA2513506) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2513506 ELISA Kit recognizes Guinea Pig MYH1. It is sometimes possible for the material contained within the vial of "MYH1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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