Guinea Pig IGFBP-7 ELISA Kit | IGFBP-7 elisa kit
Guinea pig IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGFBP-7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IGFBP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGFBP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IGFBP-7. You can calculate the concentration of IGFBP-7 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]
Uniprot Description
IGFBP7: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Defects in IGFBP7 are the cause of retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS). RAMSVPS is an autosomal recessive condition characterized by the bilateral appearance of 'beading' along the major retinal arterial trunks, with the subsequent formation of macroaneurysms. Affected individuals also have supravalvular pulmonic stenosis, often requiring surgical correction.
Protein type: Cell cycle regulation; Secreted; Cell adhesion; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q12
Cellular Component: extracellular matrix; extracellular space; extracellular region
Molecular Function: insulin-like growth factor binding; protein binding
Biological Process: negative regulation of cell proliferation; response to cortisol stimulus; response to retinoic acid; response to heat; regulation of steroid biosynthetic process; regulation of cell growth; cell adhesion; inner ear development; embryo implantation; cellular response to hormone stimulus
Disease: Retinal Arterial Macroaneurysm With Supravalvular Pulmonic Stenosis
