Human IGFBP7 ELISA Kit | IGFBP7 elisa kit

Human IGFBP7 ELISA Kit

Cat. Num. AAA825101

• Gene Names: IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• Reactivity: Human
• Applications: ELISA
• Synonyms: IGFBP7; Human IGFBP7 ELISA Kit; MAC25; PSF; Insulin-like growth factor-binding protein 7; IBP-7; IGF-binding protein 7; IGFBP-7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF; IGFBP7 elisa kit

• Reactivity: Human
• Specificity: The Human IGFBP7 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IGFBP7 proteins within the range of 78 pg/ml - 5000 pg/ml.
• Sequence Length: 279

• Applicable Applications for IGFBP7 elisa kit: Sandwich ELISA (SE)
• Samples: Cell culture supernatants, serum, plasma, urine
• Sensitivity: < 40 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP7 elisa kit

Principle of the Assay: The MyBioSource' Human IGFBP7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP7 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human IGFBP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 359465608
• NCBI GeneID: 3490
• NCBI Accession #: NP_001240764.1
• NCBI GenBank Nucleotide #: NM_001253835.1
• UniProt Accession #: Q16270; Q07822; Q53YE6; Q9UCA8; B4E1N2; B7Z9W7
• Molecular Weight: 28,860 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 7 isoform 2
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 7
• NCBI Official Symbol: IGFBP7
• NCBI Official Synonym Symbols: AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• NCBI Protein Information: insulin-like growth factor-binding protein 7; IGFBP-rP1; angiomodulin; IGF-binding protein 7; PGI2-stimulating factor; tumor-derived adhesion factor; prostacyclin-stimulating factor
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP7
• UniProt Synonym Gene Names: MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF
• UniProt Entry Name: IBP7_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]

Uniprot Description

IGFBP7: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Defects in IGFBP7 are the cause of retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS). RAMSVPS is an autosomal recessive condition characterized by the bilateral appearance of 'beading' along the major retinal arterial trunks, with the subsequent formation of macroaneurysms. Affected individuals also have supravalvular pulmonic stenosis, often requiring surgical correction.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; protein binding

Biological Process: negative regulation of cell proliferation; response to retinoic acid; response to cortisol stimulus; response to heat; regulation of steroid biosynthetic process; regulation of cell growth; cell adhesion; inner ear development; embryo implantation; cellular response to hormone stimulus

Disease: Retinal Arterial Macroaneurysm With Supravalvular Pulmonic Stenosis