Typical Testing Data/Standard Curve (for reference only)
Guinea Pig CYTOCHROME P-45017 ALPHA GENE ELISA KIT (CYP17) ELISA Kit | CYP17 elisa kit
Guinea Pig CYTOCHROME P-45017 ALPHA GENE ELISA KIT (CYP17)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CYP17 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of CYP17 in the sample, this CYP17 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus CYP17 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of CYP17 utilizes a multiclonal anti-CYP17 antibody and an CYP17-HRP conjugate. The assay sample and buffer are incubated together with CYP17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP17 concentration since CYP17 from samples and CYP17-HRP conjugate compete for the anti-CYP17 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP17 from the sample, fewer sites are left to bind CYP17-HRP conjugate. Standards of known CYP17 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of CYP17. The CYP17 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; Lipid Metabolism - C21-steroid hormone; EC 1.14.99.9; EC 4.1.2.30
Chromosomal Location of Human Ortholog: 10q24.3
Cellular Component: endoplasmic reticulum membrane; cell soma; mitochondrion; endoplasmic reticulum; axon
Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; steroid 17-alpha-monooxygenase activity; iron ion binding; heme binding; oxygen binding
Biological Process: steroid metabolic process; response to cAMP; dibenzo-p-dioxin metabolic process; progesterone metabolic process; androgen biosynthetic process; response to insecticide; Leydig cell differentiation; biphenyl metabolic process; response to methylmercury; hormone biosynthetic process; response to nutrient levels; response to drug; response to retinoic acid; adrenal gland development; hippocampus development; phthalate metabolic process; response to herbicide; ovulation; response to acetate; response to steroid hormone stimulus; xenobiotic metabolic process; response to cytokine stimulus; response to ionizing radiation; glucocorticoid biosynthetic process; steroid biosynthetic process; sex differentiation; sterol metabolic process; phenol metabolic process
Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency
Research Articles on CYP17
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Product Notes
The Guinea Pig CYP17 cyp17a1 (Catalog #AAA733826) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA733826 ELISA Kit recognizes Guinea Pig CYP17. It is sometimes possible for the material contained within the vial of "CYTOCHROME P-45017 ALPHA GENE ELISA KIT (CYP17), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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