Mouse Hedgehog Homolog, Indian (IHH) ELISA Kit | IHH elisa kit

Mouse Hedgehog Homolog, Indian (IHH) ELISA Kit

Cat. Num. AAA2704925

• Gene Names: IHH; BDA1; HHG2
• Reactivity: Mouse
• Synonyms: Hedgehog Homolog; Indian (IHH); Mouse Hedgehog Homolog; Indian (IHH) ELISA Kit; BDA1; HHG2; IHH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Indian (IHH).; No significant cross-reactivity or interference between Hedgehog Homolog, Indian (IHH) and analogues was observed.
• Sequence Length: 293

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Homolog, Indian (IHH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Homolog, Indian (IHH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IHH elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Indian (IHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Indian (IHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Indian (IHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Homolog, Indian (IHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 823671736
• NCBI GeneID: 3549
• Molecular Weight: 45,251 Da
• NCBI Official Full Name: IHH, partial
• NCBI Official Synonym Full Names: indian hedgehog
• NCBI Official Symbol: IHH
• NCBI Official Synonym Symbols: BDA1; HHG2
• NCBI Protein Information: indian hedgehog protein
• UniProt Protein Name: Indian hedgehog protein
• Protein Family: Indian hedgehog protein
• UniProt Gene Name: IHH
• UniProt Synonym Gene Names: IHH

NCBI Description

This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Nov 2015]

Uniprot Description

IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; plasma membrane; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; patched binding; peptidase activity

Biological Process: bone resorption; branching involved in blood vessel morphogenesis; cell fate specification; cell maturation; cell-cell signaling; embryonic camera-type eye morphogenesis; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic pattern specification; heart looping; in utero embryonic development; intein-mediated protein splicing; maternal process involved in female pregnancy; multicellular organism growth; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of eye pigmentation; negative regulation of immature T cell proliferation in the thymus; negative regulation of T cell differentiation in thymus; neuron development; osteoblast differentiation; pancreas development; positive regulation of alpha-beta T cell differentiation; positive regulation of collagen biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; positive regulation of T cell differentiation in thymus; positive regulation of transcription from RNA polymerase II promoter; proteoglycan metabolic process; regulation of growth; response to estradiol; response to mechanical stimulus; skeletal system development; smooth muscle development; smoothened signaling pathway; vitelline membrane formation

Disease: Acrocapitofemoral Dysplasia; Brachydactyly, Type A1