Western Blot (WB)
(Figure 1: Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate.)
Mouse GTF2B Monoclonal Antibody | anti-GTF2B antibody
GTF2B Antibody
WB: 1:500-1:2000
Western Blot (WB)
(Figure 1: Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate.)
Function: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Subunit Structure: Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2 (PubMed:8566795). Interacts with GPBP1 (By similarity). Interacts with HSF1 (via transactivation domain) (PubMed:11005381).
Similarity: Belongs to the TFIIB family.
NCBI and Uniprot Product Information
Predicted: 35 kDa
NCBI Description
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
Uniprot Description
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
Research Articles on GTF2B
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Product Notes
The GTF2B gtf2b (Catalog #AAA9602453) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GTF2B Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the GTF2B gtf2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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