Pig Glutathione S-transferase omega-1 ELISA Kit | GSTO1 elisa kit
Pig Glutathione S-transferase omega-1 (GSTO1) ELISA Kit
Gene Names
GSTO1; SPG-R; GSTO 1-1
Reactivity
Pig
Synonyms
Glutathione S-transferase omega-1; Pig Glutathione S-transferase omega-1 (GSTO1) ELISA Kit; GSTO1 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig GSTO1. No significant cross-reactivity or interference between Pig GSTO1 and analogues was observed.
Sequence Length
241
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GSTO1 elisa kit
Glutathione S-transferase omega-1 is an enzyme that encodes a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, the encoded protein acts as a small stress response protein, likely involved in cellular redox homeostasis.This protein has dehydroascorbate reductase activity and may function in the glutathione-ascorbate cycle as part of antioxidant metabolism.
GSTO1 has an N-terminal thioredoxin-like domain consisting of a central 4-stranded beta sheet flanked on one side by 2 alpha helices and on the other by a 3-10 helix. The C terminus is composed of 7 alpha helices and makes several H bonds with the N-terminal domain. GSTO1 forms a dimer and adopts the typical GST tertiary structure.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GSTO1 in samples. An antibody specific for GSTO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGSTO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSTO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSTO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
GSTO1 has an N-terminal thioredoxin-like domain consisting of a central 4-stranded beta sheet flanked on one side by 2 alpha helices and on the other by a 3-10 helix. The C terminus is composed of 7 alpha helices and makes several H bonds with the N-terminal domain. GSTO1 forms a dimer and adopts the typical GST tertiary structure.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GSTO1 in samples. An antibody specific for GSTO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGSTO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSTO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSTO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,419 Da
NCBI Official Full Name
glutathione S-transferase omega-1
NCBI Official Symbol
GSTO1
NCBI Official Synonym Symbols
SPG-R; GSTO 1-1
NCBI Protein Information
glutathione S-transferase omega-1
UniProt Protein Name
Glutathione S-transferase omega-1
Protein Family
UniProt Gene Name
GSTO1
UniProt Synonym Gene Names
MMA(V) reductase; SPG-R
Uniprot Description
Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
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Product Notes
The Pig GSTO1 gsto1 (Catalog #AAA282140) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282140 ELISA Kit recognizes Pig GSTO1. It is sometimes possible for the material contained within the vial of "Glutathione S-transferase omega-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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