Mouse gastrin-releasing peptide ELISA Kit | GRP elisa kit

Mouse Gastrin-releasing peptide, GRP ELISA Kit

Cat. Num. AAA935048

• Gene Names: Grp; BLP
• Reactivity: Mouse
• Synonyms: gastrin-releasing peptide; Mouse Gastrin-releasing peptide; GRP ELISA Kit; Mouse Gastrin-releasing peptide (GRP) ELISA kit; BN; GRP-10; preproGRP; proGRP; bombesin; neuromedin C; pre-progastrin releasing peptide; GRP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse GRP. No significant cross-reactivity or interference between mouse GRP and analogues was observed.
• Sequence Length: 146

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 39 pg/ml-2500 pg/ml
• Sensitivity: < 9.75 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 30424687
• NCBI GeneID: 225642
• NCBI Accession #: NP_778177.1
• NCBI GenBank Nucleotide #: NM_175012.3
• UniProt Accession #: Q8R1I2; Q3UTL2
• Molecular Weight: 15,659 Da
• NCBI Official Full Name: gastrin-releasing peptide preproprotein
• NCBI Official Synonym Full Names: gastrin releasing peptide
• NCBI Official Symbol: Grp
• NCBI Official Synonym Symbols: BLP
• NCBI Protein Information: gastrin-releasing peptide
• UniProt Protein Name: Gastrin-releasing peptide
• Protein Family: Gastrin-releasing peptide
• UniProt Gene Name: Grp
• UniProt Synonym Gene Names: GRP
• UniProt Entry Name: GRP_MOUSE

NCBI Description

This gene encodes a neuropeptide hormone that affects various biological processes such as neuroendocrine regulation, gastrointestinal secretion, nociception, cell proliferation and inflammation. The encoded protein undergoes proteolytic processing to generate multiple mature peptides with biological activity. [provided by RefSeq, Aug 2015]

Uniprot Description

GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity

Biological Process: neuropeptide signaling pathway